The goal of chicken embryonic spinal cord electroporation is to increase or to reduce expression levels of genes of interest in the developing spinal cord, and to assess the phenotypic consequences of these alterations on neuronal differentiation or migration. Fertilized eggs stored at 14°C are
Last updated on: 13-12-2022 - 11:23
Contact: Frédéric Clotman
Organisation: Université Catholique de Louvain (UCL)
Status: History of use, Internally validated, Published in peer reviewed journal
The in vitro micronucleus test is a genotoxicity test for the detection of micronuclei in the cytoplasm of interphase cells and has been described in detail in OECD TG 487. Micronuclei may originate from acentric chromosome fragments (i.e. lacking a centromere), or whole chromosomes that
Last updated on: 23-11-2022 - 16:31
Contact: Birgit Mertens
Organisation: Sciensano
Status: Published in peer reviewed journal, Validated by an external party (e.g. OECD, EURL ECVAM,…)
This method describes the generation of porcine testicular organoids using piglet testicular cells seeded in a testicular extracellular matrix (tECM) hydrogel. To generate the solublized tECM hydrogel, porcine immature testicular tissues (ITTs) were dissected in small fragments and decellularized in
Last updated on: 04-11-2022 - 08:20
Contact: Marc Kanbar
Organisation: Université Catholique de Louvain (UCL)
Status: Published in peer reviewed journal
E. coli is one of the organisms of choice for the production of recombinant proteins. DH5 alpha cells are commonly used for maintenance, propagation and mutation, whilst BL21(DE3) and C43(DE3) are mainly used for expression of the transgene. The advantage of C43(DE3) is that is used to produce
Last updated on: 03-11-2022 - 07:25
Contact: Jessie Neuckermans
Organisation: Vrije Universiteit Brussel (VUB)
Status: Still in development, History of use, Internally validated
We have developed a protocol for culturing primary neuron progenitor cells (NPCs) derived from mouse embryos at embryonic day 15 (E15). These cells are highly proliferative, can be subcultured and cryopreserved and can be differentiated to neurons or astrocytes. Hence, this method can greatly reduce
Last updated on: 20-10-2022 - 08:55
Contact: Roel Quintens
Organisation: Belgian Nuclear Research Centre
Status: Still in development, History of use, Internally validated
After digestion with pronase, human epithelial cells are cultured on flask and then on inserts to recapitulate a fully differentiated epithelium.
Last updated on: 19-10-2022 - 16:54
Contact: Sophie Gohy
Organisation: Cliniques universitaires Saint-Luc, UCLouvain
Status: Published in peer reviewed journal
The in vitro rumen fermentation technique is used to simulate the rumen function/fermentation. The rumen is the first compartment of the digestive tract of ruminants, in which feed - consumed by ruminants - is fermented by rumen microbes. For optimal fermentation, the rumen provides an anaerobic
Last updated on: 14-10-2022 - 11:37
Contact: Veerle Fievez
Organisation: Ghent University (UGent)
Status: Published in peer reviewed journal
Studying spermatogenesis in situ has led to the understanding that the 3D reorganization of testicular cells into an interstitial and tubular compartment is of enormous importance for germ cell differentiation. We will rely on 3D bioprinting technology which gives control over cell deposition and
Last updated on: 06-10-2022 - 14:11
Contact: Guillaume Richer
Organisation: Vrije Universiteit Brussel (VUB)
Status: Still in development, Published in peer reviewed journal
The tri-dimensional (3D) culture protocols allow isolation and culture of gastric epithelial antral and intestinal stem cells to efficiently generate organoids that recapitulate the mature pyloric and intestinal epithelium in vitro and foetal epithelial progenitors of these tissues growing as
Last updated on: 16-08-2022 - 10:57
Contact: Marie-Isabelle Garcia
Organisation: Université Libre de Bruxelles (ULB)
Status: History of use, Internally validated, Published in peer reviewed journal
The main objective of this research project is to create the first full-thickness cornea-on-chip, which comprises a 3D construct with every cellular layer of the cornea. This construct is embedded in a microfluidic chip with two channels that are continuously perfused. The epithelial side of the
Last updated on: 27-07-2022 - 09:35
Contact: Joris Van Meenen
Organisation: University of Antwerp (UAntwerpen)
Status: Still in development
