Adult intestinal stem cells are grown into closed 3D structures with protruding buds and a luminal compartment. These 3D structures are produced from the stem cells present in the base of crypts isolated from rectal mucosa samples that can be collected by suction or forceps biopsy. The crypts are

Last updated on: 22-02-2024 - 11:15

Contact: Anabela Ramalho
Organisation: Katholieke Universiteit Leuven (KUL)
Status: Published in peer reviewed journal
Intestinal organoids are cultured from intestinal biopsies obtained during routine endoscopy. The stem cell containing crypts are isolated and cultured in a 3D ECM (Matrigel) in the presence of the desired growth factors. The present stem cells will expand and give rise to all epithelial cells of

Last updated on: 11-01-2023 - 16:43

Contact: Bram Verstockt
Organisation: Katholieke Universiteit Leuven (KUL)
Status: Published in peer reviewed journal
This method uses human skin-derived precursors (hSKP) differentiated towards hepatic cells (hSKP-HPC) as a hepatic in vitro model. Exposure of these cells for 24 hours to sub-cytotoxic concentrations of acetaminophen, which is a reference hepatotoxicant, induced specific cellular responses in a

Last updated on: 26-05-2022 - 10:39

Contact: Robim Rodrigues
Organisation: Vrije Universiteit Brussel (VUB)
Status: Published in peer reviewed journal
The CALUX method is an in vitro bioassay that uses reporter gene cell lines that have been stably transfected with a luciferase reporter gene under the control of relevant receptor specific DNA response element. This enables the screening for chemicals that can bind to specific receptors and

Last updated on: 16-03-2022 - 14:47

Contact: Imke Boonen
Organisation: Vrije Universiteit Brussel (VUB)
The neutral red uptake assay is a cell viability assay that allows in vitro quantification of xenobiotic-induced cytotoxicity. The assay relies on the ability of living cells to incorporate and bind neutral red, a weak cationic dye, in lysosomes. As such, cytotoxicity is expressed as a concentration

Last updated on: 28-02-2022 - 11:04

Contact: Robim Rodrigues
Organisation: Vrije Universiteit Brussel (VUB)
Status: Published in peer reviewed journal, Validated by an external party (e.g. OECD, EURL ECVAM,…)
The cardiorespiratory model reproduces human physiology with a high level of fidelity. It is a lumped parameter model including a representation of atria, ventricles, pulmonary and systemic circulations, autonomic controls, metabolic peripheral control, ventilation and gas exchange in tissues and

Last updated on: 21-10-2021 - 10:07

Contact: Libera Fresiello
Organisation: Cannot be disclosed
Partners: Cannot be disclosed
Status: History of use, Internally validated, Published in peer reviewed journal
We aimed to optimize fibrin matrix composition in order to mimic human ovarian tissue architecture for human ovarian follicle encapsulation and grafting. Ultrastructure of fresh human ovarian cortex in age-related women (n = 3) and different fibrin formulations (F12.5/T1, F30/T50, F50/T50, F75/T75),

Last updated on: 10-09-2021 - 14:18

Organisation: Université Catholique de Louvain (UCL)
Status: Published in peer reviewed journal
Characterization of Human Oligodendroglia derived-EV-rich in sHSPs (HOG-EV-sHSPs). Establish HSPB1/B8 stably expressing cell lines for the production of sHSPs rich-EV cell culture conditioned medium. Human Oligodendroglia cell lines (HOG) were stably transduced with pLenti-HSPBs gene constructs

Last updated on: 14-08-2020 - 15:22

Contact: Joy Irobi
Organisation: University of Hasselt (UHasselt)
Status: Still in development, Internally validated, Currently submitted for further validation by an external party (e.g. OECD, EURL ECVAM,…)
We have developed and validated yeast models to study aspects related to protein folding diseases like Alzheimer's and Parkinson's disease. These models allow to gain further insight in the cellular processes involved in the etiology of these disorders and as such identify potential new bio-markers

Last updated on: 11-02-2020 - 11:17

Contact: Joris Winderickx
Organisation: Katholieke Universiteit Leuven (KUL)
Status: Published in peer reviewed journal
FIBEr (Flanders Institute for Biomechanical Experimentation) is a laboratory that investigates the mechanical properties (stiffness, strength, microstructure) of biological tissue, in casu bones, muscles, ligaments, blood vessels, etc. A quantitative characterization of the mechanical behavior of

Last updated on: 12-11-2019 - 11:45

Contact: Nele Famaey
Organisation: Katholieke Universiteit Leuven (KUL)
Partners: Katholieke Universiteit Leuven (KUL)
Status: Still in development, History of use, Internally validated