Vascular smooth muscle cell (VSMC) deficiency plays a pivotal role in aneurysm development. Unfortunately, access to native VSMCs of patients and (particularly) control individuals is extremely limited. It has been shown that iPSC-derived VSMCs recapitulate the yet known disease processes very well.

Last updated on: 03-06-2020 - 13:51

Organisation: University of Antwerp (UAntwerpen)
Status: Still in development, Internally validated
Reverse pharmacology is a high-throughput in vitro method to characterise ligand-receptor interactions. In this method, a receptor of interest is expressed in a heterologous cell line and used as a hook to fish out its ligand(s) from a library of synthetic compounds. Receptor activation is measured

Last updated on: 17-04-2020 - 09:56

Contact: Isabel Beets
Organisation: Katholieke Universiteit Leuven (KUL)
Status: Published in peer reviewed journal
Computational Fluid Dynamics (CFD) is being applied to characterize the fluid flow in different applications. CFD has obtained significant interest in both the medical and engineering community because of its non-invasive character. It can predict the fluid flow characteristics when one or multiple

Last updated on: 10-04-2020 - 09:22

Organisation: Ghent University (UGent)
Status: Still in development, Published in peer reviewed journal
Performing biopredictive dissolution tests in in vitro models that are frequently used in pharmaceutical and academic institutions and using these in vitro dissolution data as input for PBPK models to predict the systemic exposure of the drug in humans/patients.

Last updated on: 08-04-2020 - 17:29

Contact: Bart Hens
Organisation: Katholieke Universiteit Leuven (KUL)
Status: Published in peer reviewed journal
We study the influence of food parameters on the digestive kinetics of diverse nutrients throughout the upper gastrointestinal tract. For this, we use a static in vitro digestion protocol based on the standardized, consensus method recommended by the INFOGEST consortium. Briefly, the food is first

Last updated on: 31-03-2020 - 21:01

Contact: Tara Grauwet
Organisation: Katholieke Universiteit Leuven (KUL)
Partners: INFOGEST consortium
Status: Published in peer reviewed journal
The objective of this ex-vivo model is to study the initial pulp-tissue reaction of the human pulp tissue to different pulp-capping materials. Methodology: Freshly-extracted (mainly due to orthodontic reasons) healthy human teeth (impacted third molars) from young individuals (15-20 years old)

Last updated on: 24-03-2020 - 16:32

Contact: Mariano Pedano
Organisation: Katholieke Universiteit Leuven (KUL)
Partners: Aix-Marseille University
Status: History of use, Internally validated, Published in peer reviewed journal
Design and fabrication of microfluidic devices that allow manipulation and analysis of (single) cells. Droplet-based as well as digital microfluidics can be applied and are suitable for a wide variety of (non-adherent) cells. Different materials can be used for the fabrication of the microfluidic

Last updated on: 10-03-2020 - 14:54

Contact: Cannot be disclosed
Organisation: Katholieke Universiteit Leuven (KUL)
Status: Still in development, Published in peer reviewed journal
Organotypic epithelial raft cultures accurately reproduce the process of epithelial differentiation in vitro and can be prepared from normal keratinocytes, explanted epithelial tissue, or established cell lines. Normal primary human keratinocytes (PHKs) stratify and fully differentiate in a manner

Last updated on: 04-03-2020 - 14:34

Contact: Graciela Andrei
Organisation: Katholieke Universiteit Leuven (KUL)
Status: History of use, Internally validated, Published in peer reviewed journal
This white-box model uses energy partitioning throughout the lifetime of dairy animals (growth, lactation, gestation, ...) to simulate reproduction performance, lifetime length, production performance etc. The method is developed by dr. Olivier Martin at INRAE, MoSAR, Paris.

Last updated on: 04-03-2020 - 14:23

Contact: Cannot be disclosed
Organisation: Cannot be disclosed
Status: History of use, Published in peer reviewed journal
Brain from treated and not treated mice is collected after death. Tissue is fixed in 4% PFA for 4 days. After specific cutting (ex. L and R hemisphere) tissue is placed in a cassette. Cassette is placed in Tissue Processor (where water from the tissue is removed and replaced with paraffin). Brain is

Last updated on: 04-03-2020 - 14:20

Contact: Cannot be disclosed
Organisation: Katholieke Universiteit Leuven (KUL)
Status: History of use, Internally validated