UHDR irradiations show healthy tissue sparing effect known as the FLASH effect. Since 2014, the FLASH effect is investigated worldwide to understand how it works and how to trigger it. The FLASH effect is defined as an in vivo effect. However, in vivo models are often expensive and time-consuming.

Last updated on: 05-09-2023 - 08:45

Contact: Lucas Schoenauen
Organisation: Université de Namur (UNamur)
Status: Still in development
Thyroid hormone system disruption (THSD) has detrimental effects on both human and environmental health. As a rising number of chemicals are reported to interfere with the thyroid hormone system, there is an increasing need for fast and reliable evaluation methods to test for THSD. Currently,

Last updated on: 27-04-2023 - 16:41

Contact: Cannot be disclosed
Organisation: Cannot be disclosed
Partners: Cannot be disclosed
Status: Currently submitted for further validation by an external party (e.g. OECD, EURL ECVAM,…)
The goal of chicken embryonic spinal cord electroporation is to increase or to reduce expression levels of genes of interest in the developing spinal cord, and to assess the phenotypic consequences of these alterations on neuronal differentiation or migration. Fertilized eggs stored at 14°C are

Last updated on: 13-12-2022 - 11:23

Organisation: Université Catholique de Louvain (UCL)
Status: History of use, Internally validated, Published in peer reviewed journal
Amoebae are natural eukaryotic predators of bacteria, yeasts, fungi and they are ubiquiste. They are excellent and easy-to-use cellular infection models, as they allow to co-cultivate any organisms in a broad range of infection medium, compatible with high quality microscopy techniques,

Last updated on: 16-03-2022 - 14:45

Organisation: Vrije Universiteit Brussel (VUB)
Status: Internally validated, Published in peer reviewed journal
A calculated number of bacteria is injected through the pro-legs of the larvae. As several entry points are present, this allows to inject virulent bacteria and potential antimicrobial compounds within the same host. The survival of the larvae is scored over time (days post inoculation) until about

Last updated on: 16-03-2022 - 14:43

Organisation: Vrije Universiteit Brussel (VUB)
Status: Internally validated, Published in peer reviewed journal
The nematode Caenorhabditis elegans provides a powerful model system to study fundamental working mechanisms of the nervous system in a living animal. It's main advantages are its compact nervous system that has been fully mapped, its short generation time and amenability for genetic research. As a

Last updated on: 17-04-2020 - 09:55

Contact: Isabel Beets
Organisation: Katholieke Universiteit Leuven (KUL)
Status: Published in peer reviewed journal
The lab routinely use C. elegans culture as genetic model. We explore the development and the ageing of the neuromuscular system. The methods used regularly in our lab include molecular biology, generation of transgenic animals or mutants, crossings, genetic screen, calcium imaging, fluorescence

Last updated on: 02-12-2019 - 13:54

Contact: patrick laurent
Organisation: Université Libre de Bruxelles (ULB)
Status: Internally validated, Published in peer reviewed journal
The Galleria mellonella assay makes use of larvae of the Greater Wax Moth. G. mellonella larvae are injected with a small amount of microorganisms, and survival can easily be followed for up to 1 week. This allows to compare virulence between different organisms. Alternatively, after injection of

Last updated on: 08-11-2019 - 13:58

Contact: Tom Coenye
Organisation: Ghent University (UGent)
Status: Published in peer reviewed journal
The C. elegans assay can be used to study the virulence of various microorganisms, and can be used to evaluate the antimicrobial effect of drugs or drug candidates. The two main outcome parameters are (i) survival of C. elegans and (ii) microbial load.

Last updated on: 08-11-2019 - 13:57

Contact: Tom Coenye
Organisation: Ghent University (UGent)
Status: Published in peer reviewed journal
The chorioallantoic membrane (CAM) is a highly vascularized membrane which results from the fusion of two extra-embryonic membranes, namely the chorion and the allantois. The CAM is easily accessible for manipulation and imaging, but methodologies differ whether the chicken embryo stays within its

Last updated on: 08-11-2019 - 13:27

Contact: Hanna Tay
Organisation: Ghent University (UGent)
Status: Internally validated