In this method, it is possible to use active/viable animal or human brain slices / cells (normal or disease model) to study the effects of different drugs on brain cells (neuron or glia) in diverse brain region.

Last updated on: 10-09-2019 - 09:50

Contact: Surajit Sahu
Organisation: Vrije Universiteit Brussel
Status: History of use, Published in peer reviewed journal

Prototyping and replication (small series production) of microfluidic or optofluidic devices, in thermoplastic polymers or in glass. 3D nanoprinting is also available to produce microscaffolds, possibly within microfluidic channels.

Last updated on: 29-08-2019 - 15:06

Contact: Jürgen Van Erps
Organisation: Vrije Universiteit Brussel
Status: Internally validated, Published in peer reviewed journal

Recent improvements on the structural aspects of organ-on-chips pave the way towards a large-scale application. As such soon the number of read-out instruments that are in operation in parallel will need to drastically increase. Unfortunately, standard read-out equipment is bulky, complex and

Last updated on: 27-08-2019 - 18:22

Organisation: Vrije Universiteit Brussel
Status: Still in development

Loss of acinar differentiation drives pancreatic cancer. An established human in vitro experimental model is used in our lab to study this process. Pancreatic exocrine cells from human donors are placed in suspension culture in Advanced RPMI medium supplemented with 5% heat-inactivated fetal

Last updated on: 21-08-2019 - 11:36

Contact: Elyne Backx
Organisation: Vrije Universiteit Brussel
Status: Published in peer reviewed journal

This method describes the steps to go from a liver to a decellularized matrix. It uses mild and strong detergents to destroy cells and keep the extracellular matrix intact. This matrix can then be used for a variety of purposes, including (but not limited to) repopulation, basis for coating and

Last updated on: 08-05-2019 - 14:33

Contact: Paul Claes
Organisation: Vrije Universiteit Brussel
Status: Published in peer reviewed journal

This method describes the steps from a living rat to a single cell solution of primary hepatocytes. This requires surgery on the lab animal, a perfusion with buffer solution, a digestion with collagenase and a filtration step to obtain primary hepatocytes.

Last updated on: 08-05-2019 - 14:33

Contact: Paul Claes
Organisation: Vrije Universiteit Brussel

Rat liver epithelial cells (rLEC) can be isolated from 8-day old male Sprague-Dawley rats. Briefly, small fragments of neonatal rat livers are incubated for 15 minutes with 4-(2-hydroxyethyl)-1-piperazine-ethanesulfonic acid (HEPES) buffered trypsin solution [0.25 % (v/v)] and washed twice with

Last updated on: 08-05-2019 - 14:33

Contact: Joery De Kock
Organisation: Vrije Universiteit Brussel
Status: History of use, Internally validated, Published in peer reviewed journal

This method describes a well-known optimised human in vitro model of drug-induced cholestasis. Cryopreserved primary human hepatocytes are cultured between two layers of extracellular matrix scaffold, which will delay dedifferentiation and allows to restore cell-extracellular matrix interactions.

Last updated on: 02-04-2019 - 10:45

Contact: Eva Gijbels
Organisation: Vrije Universiteit Brussel
Status: Still in development

This method uses human skin-derived precursors (hSKP) differentiated towards hepatic cells (hSKP-HPC) as an hepatic in vitro model. Exposure of these cells for 24 hours to sub-cytotoxic concentrations of acetaminophen, which is a reference hepatotoxicant, induced specific cellular responses in a

Last updated on: 27-03-2019 - 16:43

Contact: Robim Rodrigues
Organisation: Vrije Universiteit Brussel
Status: Published in peer reviewed journal