The feed undergoes an in vitro enzymatic hydrolysis simulating the digestion in the upper digestive tract followed by a dialysis mimicking the intestinal absorption. Finally, an in vitro batch fermentation is performed and simulates the fermentation occurring in the hindgut. The ingredients, ground

Last updated on: 16-07-2020 - 15:04

Contact: Nadia Everaert
Organisation: Liège Université
Status: Published in peer reviewed journal
Prebiotics are used by the gut microbiota creating metabolites that exert a positive effect on the host (postbiotics). Prebiotics are available on the market for human and animal consumption and may induce differential effects on the host's health and metabolism. They can be tested by in vitro

Last updated on: 16-07-2020 - 15:02

Contact: Nadia Everaert
Organisation: Liège Université
Status: Published in peer reviewed journal
Cardiomyocytes derived from induced pluripotent stem cells (iPSC-CMs) offer an attractive platform for cardiovascular research, including disease modeling, drug toxicity testing and development of regenerative therapies. Patient-specific iPSC-CMs are very useful to study disease pathogenesis and

Last updated on: 30-06-2020 - 14:13

Contact: Maaike Alaerts
Organisation: University of Antwerp
Status: Still in development, Internally validated
Vascular smooth muscle cell (VSMC) deficiency plays a pivotal role in aneurysm development. Unfortunately, access to native VSMCs of patients and (particularly) control individuals is extremely limited. It has been shown that iPSC-derived VSMCs recapitulate the yet known disease processes very well.

Last updated on: 03-06-2020 - 13:51

Organisation: University of Antwerp
Status: Still in development, Internally validated
This method is a straightforward, colorimetric, and inexpensive high-throughput screening system in bacteria which depends on the activity of human HPD. This screening assay is based on the formation and accumulation of a melanin-like ochronotic pigment which has a characteristic brown color. In the

Last updated on: 26-05-2020 - 11:30

Organisation: Vrije Universiteit Brussel, RWTH Aachen
Partners: RWTH Aachen
Status: Published in peer reviewed journal
Reverse pharmacology is a high-throughput in vitro method to characterise ligand-receptor interactions. In this method, a receptor of interest is expressed in a heterologous cell line and used as a hook to fish out its ligand(s) from a library of synthetic compounds. Receptor activation is measured

Last updated on: 17-04-2020 - 09:56

Contact: Isabel Beets
Organisation: KU Leuven
Status: Published in peer reviewed journal
We study the influence of food parameters on the digestive kinetics of diverse nutrients throughout the upper gastrointestinal tract. For this, we use a static in vitro digestion protocol based on the standardized, consensus method recommended by the INFOGEST consortium. Briefly, the food is first

Last updated on: 31-03-2020 - 21:01

Contact: Tara Grauwet
Organisation: KU Leuven
Partners: INFOGEST consortium
Status: Published in peer reviewed journal
The GENOMARK tool in metabolically competent human HepaRG cells is based on the transcriptomic results obtained with 12 genotoxic (in vivo positive results) and 12 non-genotoxic (in vivo negative results) reference compounds. Genotoxic compounds were selected to cover different mechanisms of action

Last updated on: 27-03-2020 - 17:23

Organisation: Sciensano
Partners: Vrije Universiteit Brussel
Status: Internally validated, Published in peer reviewed journal
The in vitro micronucleus test is a genotoxicity test for the detection of micronuclei in the cytoplasm of interphase cells and has been described in detail in OECD TG 487. Micronuclei may originate from acentric chromosome fragments (i.e. lacking a centromere), or whole chromosomes that are unable

Last updated on: 27-03-2020 - 15:12

Organisation: Sciensano
Status: Published in peer reviewed journal, Validated by an external party (e.g. OECD, EURL ECVAM,…)
The neutral red uptake (NRU) assay provides a quantitative measurement of the number of viable cells. The test is based on the ability of living cells to take up and bind neutral red (NR), a dye which easily penetrates cell membranes via non-ionic diffusion and accumulates in the lysosomes.

Last updated on: 27-03-2020 - 14:53

Contact: Roel Anthonissen
Organisation: Sciensano
Status: History of use, Published in peer reviewed journal