We used a 7-stage protocol to generate beta cells from human Induced Pluripotent Stem Cells (iPSC) and evaluated whether these cells are responsive to the pro-inflammatory cytokines (IFNγ, IL-1β, or IFNα) that play a role in type 1 diabetes (T1D). Our data show that human iPSC-derived beta cells
Last updated on: 09-03-2021 - 21:30
Contact: Decio L. Eizirik
Organisation: Université Libre de Bruxelles (ULB)
Status: Published in peer reviewed journal
The ERα CALUX transactivation assay is described in Annex 4 of the OECD TG 455. It uses the human U2OS cell line to detect estrogenic agonist and antagonist activity mediated through human estrogen receptor alpha (hERα).This test method is specifically designed to detect hERα-mediated
Last updated on: 04-03-2021 - 16:33
Contact: Cannot be disclosed
Organisation: Vlaamse Instelling voor Technologisch Onderzoek (VITO)
Status: Validated by an external party (e.g. OECD, EURL ECVAM,…)
The method has been fully described in OECD TG 236 and is intended to determine the acute or lethal toxicity of chemicals on embryonic stages of fish (Danio rerio). Newly fertilized zebrafish eggs are exposed to the test chemical for a period of 96 hrs. Every 24 hrs. Twenty embryos (one embryo per
Last updated on: 04-03-2021 - 16:29
Contact: Hilda Witters
Organisation: Vlaamse Instelling voor Technologisch Onderzoek (VITO)
Status: Published in peer reviewed journal, Validated by an external party (e.g. OECD, EURL ECVAM,…)
The Bovine Corneal Opacity and Permeability test method (BCOP) has been fully described in OECD TG 437. It is an in vitro test method that can be used to identify chemicals (substances or mixtures) as either 1) causing “serious eye damage” (category 1 of the Globally Harmonised System for the
Last updated on: 04-03-2021 - 16:26
Contact: Sandra Verstraelen
Organisation: Vlaamse Instelling voor Technologisch Onderzoek (VITO)
Status: Validated by an external party (e.g. OECD, EURL ECVAM,…)
Four validated test methods using commercially available RhCE models are included in this Test Guideline 492. Here we focus on the SkinEthic Human Corneal Epithelium (HCE) Eye Irritation Test (EIT). It is an in vitro procedure allowing the identification of chemicals (substances and mixtures) not
Last updated on: 04-03-2021 - 16:19
Contact: Sandra Verstraelen
Organisation: Vlaamse Instelling voor Technologisch Onderzoek (VITO)
Status: Validated by an external party (e.g. OECD, EURL ECVAM,…)
This method has been described in detail in OECD TG 431 and allows the identification of non-corrosive and corrosive substances and mixtures in accordance with the UN GHS. This method further supports the sub-categorisation of corrosive substances and mixtures into optional Subcategory 1A, in
Last updated on: 04-03-2021 - 16:15
Contact: Sandra Verstraelen
Organisation: Vlaamse Instelling voor Technologisch Onderzoek (VITO)
Status: Validated by an external party (e.g. OECD, EURL ECVAM,…)
In order to find a solution for the center-weighted opacity reading associated with the OP-KIT opacitometer, a prototype of a laser light-based opacitometer (PLLBO) allowing better measurement of opacities was developed (Van Goethem et al., 2010; Annex 1). The technical optimization and optical
Last updated on: 04-03-2021 - 16:05
Contact: An Van Rompay
Organisation: Vlaamse Instelling voor Technologisch Onderzoek (VITO)
Status: Currently submitted for further validation by an external party (e.g. OECD, EURL ECVAM,…)
We have established organoid cultures from patient-derived ovarian cancer (OC), in particular from the most prevalent high-grade serous ovarian cancer (HGSOC). Testing multiple culture medium components identified neuregulin-1 (NRG1) as key factor in maximizing OC organoid development and growth,
Last updated on: 28-01-2021 - 11:08
Contact: Hugo Vankelecom
Organisation: Katholieke Universiteit Leuven (KUL)
Status: Published in peer reviewed journal
We have developed multiple organoid models from a broad spectrum of endometrial pathologies that capture endometrial disease diversity and will provide powerful research models and drug screening and discovery tools. Organoids from endometriosis show disease-associated traits and cancer-linked
Last updated on: 28-01-2021 - 11:08
Contact: Hugo Vankelecom
Organisation: Katholieke Universiteit Leuven (KUL)
Status: Published in peer reviewed journal
We have established organoids from mouse pituitary with the aim to generate a novel research model to study pituitary stem cell biology in both healthy and diseased glands. The organoids originated from the pituitary cells expressing the stem cell marker SOX2, were long-term expandable, displayed a
Last updated on: 28-01-2021 - 11:08
Contact: Hugo Vankelecom
Organisation: Katholieke Universiteit Leuven (KUL)
Status: Published in peer reviewed journal
