Reverse pharmacology is a high-throughput in vitro method to characterise ligand-receptor interactions. In this method, a receptor of interest is expressed in a heterologous cell line and used as a hook to fish out its ligand(s) from a library of synthetic compounds. Receptor activation is measured
Last updated on: 17-04-2020 - 09:56
Contact: Isabel Beets
Organisation: Katholieke Universiteit Leuven (KUL)
Status: Published in peer reviewed journal
We study the influence of food parameters on the digestive kinetics of diverse nutrients throughout the upper gastrointestinal tract. For this, we use a static in vitro digestion protocol based on the standardized, consensus method recommended by the INFOGEST consortium. Briefly, the food is first
Last updated on: 31-03-2020 - 21:01
Contact: Tara Grauwet
Organisation: Katholieke Universiteit Leuven (KUL)
Partners: INFOGEST consortium
Status: Published in peer reviewed journal
The objective of this ex-vivo model is to study the initial pulp-tissue reaction of the human pulp tissue to different pulp-capping materials.
Methodology: Freshly-extracted (mainly due to orthodontic reasons) healthy human teeth (impacted third molars) from young individuals (15-20 years old)
Last updated on: 24-03-2020 - 16:32
Contact: Mariano Pedano
Organisation: Katholieke Universiteit Leuven (KUL)
Partners: Aix-Marseille University
Status: History of use, Internally validated, Published in peer reviewed journal
Retinal gene delivery via intravitreal injection is hampered by various physiological barriers present in the eye of which the vitreoretinal (VR) interface represents the most serious hurdle. We present a retinal explant model especially designed to study the role of this interface as a barrier for
Last updated on: 24-03-2020 - 16:26
Contact: Katrien Remaut
Organisation: Ghent University (UGent)
Status: Published in peer reviewed journal
Design and fabrication of microfluidic devices that allow manipulation and analysis of (single) cells. Droplet-based as well as digital microfluidics can be applied and are suitable for a wide variety of (non-adherent) cells. Different materials can be used for the fabrication of the microfluidic
Last updated on: 10-03-2020 - 14:54
Contact: Cannot be disclosed
Organisation: Katholieke Universiteit Leuven (KUL)
Status: Still in development, Published in peer reviewed journal
Mononuclear cells are flushed from the bone marrow using a 25G needle. After centrifugation, the cells are resuspended in 50mL pre-heated DMEM containing 20ng/ml M-CSF. The cells are cultured in 10cm petri dishes for 7 days. At days 2,4 and 6, the medium is renewed after washing away non-adherent
Last updated on: 10-03-2020 - 10:17
Contact: Cannot be disclosed
Organisation: Ghent University (UGent)
Partners: Ghent University hospital (UZ Gent)
Status: Published in peer reviewed journal
Organotypic epithelial raft cultures accurately reproduce the process of epithelial differentiation in vitro and can be prepared from normal keratinocytes, explanted epithelial tissue, or established cell lines. Normal primary human keratinocytes (PHKs) stratify and fully differentiate in a manner
Last updated on: 04-03-2020 - 14:34
Contact: Graciela Andrei
Organisation: Katholieke Universiteit Leuven (KUL)
Status: History of use, Internally validated, Published in peer reviewed journal
Brain from treated and not treated mice is collected after death. Tissue is fixed in 4% PFA for 4 days. After specific cutting (ex. L and R hemisphere) tissue is placed in a cassette. Cassette is placed in Tissue Processor (where water from the tissue is removed and replaced with paraffin). Brain is
Last updated on: 04-03-2020 - 14:20
Contact: Cannot be disclosed
Organisation: Katholieke Universiteit Leuven (KUL)
Status: History of use, Internally validated
Peripheral blood mononuclear cells (PBMC) are isolated from the peripheral blood using Ficoll gradient centrifugation. The PBMC are then stained with fluorescently-labelled monoclonal antibodies directed against cell surface or intracellular proteins that can be used to identify specific immune cell
Last updated on: 03-03-2020 - 11:46
Contact: Cannot be disclosed
Organisation: University of Hasselt (UHasselt)
Status: Published in peer reviewed journal
Since adult zebrafish retinal ganglion cells (RGCs) can fully regenerate upon axonal injury, these neurons form the ideal subject to study what is driving the recovery process. The use of an adult zebrafish retinal cell culture in a microfluidic set-up enables to create a neuronal network, mimicking
Last updated on: 02-03-2020 - 16:01
Contact: Annelies Van Dyck
Organisation: Katholieke Universiteit Leuven (KUL)
Status: Still in development
