Follicle Biology Laboratory has developed a well characterized and standardized MOUSE in vitro follicle culture (IFC) system. In this system, early stage ovarian follicles are cultured in vitro under physiological hormone concentrations up to fertilizable and developmentally competent mature oocytes

Last updated on: 16-03-2022 - 14:32

Contact: Ellen Anckaert
Organisation: Vrije Universiteit Brussel (VUB)
Status: History of use, Internally validated, Published in peer reviewed journal
In this method, it is possible to use active/viable animal or human brain slices / cells (normal or disease model) to study the effects of different drugs on brain cells (neuron or glia) in diverse brain region.

Last updated on: 16-03-2022 - 14:28

Contact: Surajit Sahu
Organisation: Vrije Universiteit Brussel (VUB)
Status: History of use, Published in peer reviewed journal
Recent improvements on the structural aspects of organ-on-chips pave the way towards a large-scale application. As such soon the number of read-out instruments that are in operation in parallel will need to drastically increase. Unfortunately, standard read-out equipment is bulky, complex and

Last updated on: 16-03-2022 - 14:23

Organisation: Vrije Universiteit Brussel (VUB)
Status: Still in development
Loss of acinar differentiation drives pancreatic cancer. An established human in vitro experimental model is used in our lab to study this process. Pancreatic exocrine cells from human donors are placed in suspension culture in Advanced RPMI medium supplemented with 5% heat-inactivated fetal bovine

Last updated on: 16-03-2022 - 14:19

Contact: Elyne Backx
Organisation: Vrije Universiteit Brussel (VUB)
Status: Published in peer reviewed journal
We developed a high-throughput live-cell imaging-based organoid analysis platform, Organoid Brightfield Identification-based Therapy Screening (Orbits), that allows for kinetic monitoring of organoid growth and drug responses in routine extracellular matrix domes, high-throughput 384-well

Last updated on: 16-03-2022 - 13:59

Contact: Christophe Deben
Organisation: University of Antwerp (UAntwerpen)
Status: Internally validated
The neutral red uptake assay is a cell viability assay that allows in vitro quantification of xenobiotic-induced cytotoxicity. The assay relies on the ability of living cells to incorporate and bind neutral red, a weak cationic dye, in lysosomes. As such, cytotoxicity is expressed as a concentration

Last updated on: 28-02-2022 - 11:04

Contact: Robim Rodrigues
Organisation: Vrije Universiteit Brussel (VUB)
Status: Published in peer reviewed journal, Validated by an external party (e.g. OECD, EURL ECVAM,…)
The GENOMARK tool in metabolically competent human HepaRG cells is based on the transcriptomic results obtained with 12 genotoxic (in vivo positive results) and 12 non-genotoxic (in vivo negative results) reference compounds. Genotoxic compounds were selected to cover different mechanisms of action

Last updated on: 28-02-2022 - 11:03

Contact: Anouck Thienpont
Organisation: Sciensano, Vrije Universiteit Brussel (VUB)
Status: Internally validated, Published in peer reviewed journal
By the use of monolayer cultures as an in vitro system, the effects of drugs on CYP3A activity can be evaluated. It relies on the use of a luminogenic CYP3A substrate, namely luciferin-6′-pentafluorobenzylether (luciferin- PFBE). Upon biotransformation by CYP3A, luciferin-PFBE is converted into

Last updated on: 28-02-2022 - 11:03

Contact: Steven Branson
Organisation: Vrije Universiteit Brussel (VUB)
Status: History of use
Liver functionality can be monitored by the urea synthesis. In culture medium the measurement of urea synthesis relies on a chromogenic reagent that specifically forms a colored complex with urea. The concentration of this complex between urea, o-phthalaldehyde and N-(1-naphthyl) ethylenediamine can

Last updated on: 28-02-2022 - 11:03

Contact: Steven Branson
Organisation: Vrije Universiteit Brussel (VUB)
Status: History of use
Albumin secretion in the supernatant of in vitro cultures can be measured by an enzyme-linked immunosorbent assay. The assay is based upon a quantitative sandwich enzyme immunoassay technique and measures albumin in the culture medium of primary hepatocytes and/or hepatocyte like cells. Standards

Last updated on: 28-02-2022 - 11:02

Contact: Steven Branson
Organisation: Vrije Universiteit Brussel (VUB)
Status: History of use