The protocol is aimed at developing primary human breast organoids that have a morphology similar to the one observed in the in vivo breast. This morphology encompasses a complex network organization composed of interconnected branches that terminate in TDLU-like structures. The organoids are

Last updated on: 07-11-2022 - 11:24

Organisation: Katholieke Universiteit Leuven (KUL), Oncology - KU Leuven
Status: Still in development, History of use, Published in peer reviewed journal
The protocol is aimed at developing primary mammary gland organoids that have a morphology similar to the one of the in vivo breast, which is organized as a complex network of interconnected branches. The organoids are derived from the mouse mammary gland by mechanical dissociation and enzymatic

Last updated on: 07-11-2022 - 11:08

Organisation: Oncology - KU Leuven
Status: History of use, Published in peer reviewed journal
This method describes the generation of porcine testicular organoids using piglet testicular cells seeded in a testicular extracellular matrix (tECM) hydrogel. To generate the solublized tECM hydrogel, porcine immature testicular tissues (ITTs) were dissected in small fragments and decellularized in

Last updated on: 04-11-2022 - 08:20

Contact: Marc Kanbar
Organisation: Université Catholique de Louvain (UCL)
Status: Published in peer reviewed journal
E. coli is one of the organisms of choice for the production of recombinant proteins. DH5 alpha cells are commonly used for maintenance, propagation and mutation, whilst BL21(DE3) and C43(DE3) are mainly used for expression of the transgene. The advantage of C43(DE3) is that is used to produce

Last updated on: 03-11-2022 - 07:25

Organisation: Vrije Universiteit Brussel (VUB)
Status: Still in development, History of use, Internally validated
We have developed a protocol for culturing primary neuron progenitor cells (NPCs) derived from mouse embryos at embryonic day 15 (E15). These cells are highly proliferative, can be subcultured and cryopreserved and can be differentiated to neurons or astrocytes. Hence, this method can greatly reduce

Last updated on: 20-10-2022 - 08:55

Contact: Roel Quintens
Organisation: Belgian Nuclear Research Centre
Status: Still in development, History of use, Internally validated
The in vitro rumen fermentation technique is used to simulate the rumen function/fermentation. The rumen is the first compartment of the digestive tract of ruminants, in which feed - consumed by ruminants - is fermented by rumen microbes. For optimal fermentation, the rumen provides an anaerobic

Last updated on: 14-10-2022 - 11:37

Contact: Veerle Fievez
Organisation: Ghent University (UGent)
Status: Published in peer reviewed journal
Studying spermatogenesis in situ has led to the understanding that the 3D reorganization of testicular cells into an interstitial and tubular compartment is of enormous importance for germ cell differentiation. We will rely on 3D bioprinting technology which gives control over cell deposition and

Last updated on: 06-10-2022 - 14:11

Contact: Guillaume Richer
Organisation: Vrije Universiteit Brussel (VUB)
Status: Still in development, Published in peer reviewed journal
The tri-dimensional (3D) culture protocols allow isolation and culture of gastric epithelial antral and intestinal stem cells to efficiently generate organoids that recapitulate the mature pyloric and intestinal epithelium in vitro and foetal epithelial progenitors of these tissues growing as

Last updated on: 16-08-2022 - 10:57

Organisation: Université Libre de Bruxelles (ULB)
Status: History of use, Internally validated, Published in peer reviewed journal
The main objective of this research project is to create the first full-thickness cornea-on-chip, which comprises a 3D construct with every cellular layer of the cornea. This construct is embedded in a microfluidic chip with two channels that are continuously perfused. The epithelial side of the

Last updated on: 27-07-2022 - 09:35

Contact: Joris Van Meenen
Organisation: University of Antwerp (UAntwerpen)
Status: Still in development