Experiments were conducted using cortical cells that contains glutamatergic and gabaergic neurons and glia. Primary neurons were freshly dissociated from embryonic E18-19 rat cortices as described previously and plated onto 48-well MEA plates (Maestro system, Axion Biosystems). HOne day before

Last updated on: 26-01-2021 - 17:27

Contact: Cannot be disclosed
Organisation: Janssen Pharma of JNJ
Status: Internally validated, Published in peer reviewed journal
Cell Culture and Reagents hiPSC-CMs can be obtained commercially either as living pre-plated cells seeded onto fibronectin-coated 96-well mClear plates (Greiner Bio-One, No. 655090) or can be plated in house at a density (~25,000 cells/well) suited to forming a confluent synchronously beating mono

Last updated on: 26-11-2019 - 13:17

Contact: Ard Teisman
Organisation: Janssen Pharma of JNJ
Status: Internally validated, Published in peer reviewed journal, Validated by an external party (e.g. OECD, EURL ECVAM,…)