The in vitro micronucleus test is a genotoxicity test for the detection of micronuclei in the cytoplasm of interphase cells and has been described in detail in OECD TG 487. Micronuclei may originate from acentric chromosome fragments (i.e. lacking a centromere), or whole chromosomes that are unable

Last updated on: 27-03-2020 - 15:12

Organisation: Sciensano
Status: Published in peer reviewed journal, Validated by an external party (e.g. OECD, EURL ECVAM,…)
The Ames test is a short-term bacterial reverse gene mutation assay specifically designed to detect a wide range of chemical substances that can produce genetic damage that leads to permanent gene mutations. The test has been described in detail in OECD TG 471 and employs several histidine dependent

Last updated on: 27-03-2020 - 12:26

Contact: Roel Anthonissen
Organisation: Sciensano
Status: Published in peer reviewed journal, Validated by an external party (e.g. OECD, EURL ECVAM,…)
In order to find a solution for the center-weighted opacity reading associated with the OP-KIT opacitometer, a prototype of a laser light-based opacitometer (PLLBO) allowing better measurement of opacities was developed (Van Goethem et al., 2010; Annex 1). The technical optimization and optical

Last updated on: 06-01-2020 - 16:59

Contact: An Van Rompay
Organisation: VITO
Status: Currently submitted for further validation by an external party (e.g. OECD, EURL ECVAM,…)
In view of safety of pregnant women, a promising in vitro zebrafish embryo developmental toxicity assay has been developed to test pharmaceutical and chemical compounds for their teratogenic potential. The protocol deals with exposing zebrafish embryos to a range of compound concentrations at 28°C

Last updated on: 06-01-2020 - 16:55

Organisation: University of Antwerp
Status: Still in development, Published in peer reviewed journal
Cell Culture and Reagents hiPSC-CMs can be obtained commercially either as living pre-plated cells seeded onto fibronectin-coated 96-well mClear plates (Greiner Bio-One, No. 655090) or can be plated in house at a density (~25,000 cells/well) suited to forming a confluent synchronously beating mono

Last updated on: 26-11-2019 - 13:17

Contact: Ard Teisman
Organisation: Janssen Pharma of JNJ
Status: Internally validated, Published in peer reviewed journal, Validated by an external party (e.g. OECD, EURL ECVAM,…)
Epigenetics have taken centre stage in the study of diseases such as cancer, diabetes, and neurodegeneration. A new field is emerging that targets epigenetic modifications for therapeutic intervention: pharmacoepigenetics. Particularly in oncology, inhibitors of epigenetic-modifying proteins, i.e.

Last updated on: 12-11-2019 - 11:34

Contact: Maarten Dhaenens
Organisation: Ghent University
Status: Still in development, Internally validated, Published in peer reviewed journal
The DPRA is an in chemico method which quantifies the remaining concentration of cysteine- or lysine-containing peptide following 24 hours incubation with the test chemical at 25 +/-2,5ºC. The synthetic peptides contain phenylalanine to aid in the detection. Relative peptide concentration is

Last updated on: 08-11-2019 - 10:21

Contact: bart Desmedt
Organisation: Sciensano
Partners: Sciensano
Status: History of use, Internally validated, Validated by an external party (e.g. OECD, EURL ECVAM,…)
The monocyte-activation test (MAT) is used to detect or quantify substances that activate human monocytes or monocytic cells to release endogenous mediators such as pro-inflammatory cytokines, for example tumour necrosis factor alpha (TNFα), interleukin-1 beta (IL-1β) and interleukin-6 (IL-6). These

Last updated on: 08-11-2019 - 10:13

Contact: Celine Vanhee
Organisation: Sciensano
Status: Still in development, History of use
E. coli is one of the organisms of choice for the production of recombinant proteins. DH5 alpha cells are commonly used for maintenance, propagation and mutation, whilst BL21(DE3) and C43(DE3) are mainly used for expression of the transgene. The advantage of C43(DE3) is that is used to produce

Last updated on: 08-11-2019 - 09:28

Organisation: Vrije Universiteit Brussel
Status: Still in development, History of use, Internally validated
The neutral red uptake assay is a cell viability assay that allows in vitro quantification of xenobiotic-induced cytotoxicity. The assay relies on the ability of living cells to incorporate and bind neutral red, a weak cationic dye, in lysosomes. As such, cytotoxicity is expressed as a concentration

Last updated on: 08-11-2019 - 09:17

Contact: Robim Rodrigues
Organisation: Vrije Universiteit Brussel
Status: Published in peer reviewed journal, Validated by an external party (e.g. OECD, EURL ECVAM,…)