MELN cells [provided by INSERM, Montpellier, FR; Balaguer et al. (1999)] are oestrogen-sensitive human breast cancer cells (MCF-7) stably transfected with the oestrogen-responsive gene (ERE-βGlo-Luc-SVNeo) carried by integrated plasmids. A standard set-up has been developed to expose MELN cells

Last updated on: 28-01-2020 - 16:39

Contact: Cannot be disclosed
Organisation: VITO
Status: History of use, Internally validated, Published in peer reviewed journal
The rainbow trout gill cell line assay quantifies cell viability using fluorescent measurements for metabolic activity (Alamar Blue, AB), cell membrane integrity (5-CarboxyFluorescein DiAcetate AcetoxyMethyl ester, CFDA-AM) and lysosomal membrane integrity (Neutral Red, NR). Chemicals are added to

Last updated on: 20-01-2020 - 10:26

Contact: Cannot be disclosed
Organisation: VITO
Status: History of use, Internally validated, Published in peer reviewed journal
In order to find a solution for the center-weighted opacity reading associated with the OP-KIT opacitometer, a prototype of a laser light-based opacitometer (PLLBO) allowing better measurement of opacities was developed (Van Goethem et al., 2010; Annex 1). The technical optimization and optical

Last updated on: 06-01-2020 - 16:59

Contact: An Van Rompay
Organisation: VITO
Status: Currently submitted for further validation by an external party (e.g. OECD, EURL ECVAM,…)
VITO can offer expertise in animal-free methods for inhalation testing. VITO has an Air-Liquid Interface (ALI) platform with three ALI exposure modules: two commercial systems for bronchial studies and one in-house developed system for lower airway studies. At VITO ALI exposure modules, aerosol

Last updated on: 08-11-2019 - 14:08

Organisation: VITO
Partners: VITO
Status: History of use, Internally validated, Published in peer reviewed journal