Studying spermatogenesis in situ has led to the understanding that the 3D reorganization of testicular cells into an interstitial and tubular compartment is of enormous importance for germ cell differentiation. We will rely on 3D bioprinting technology which gives control over cell deposition and

Last updated on: 08-11-2019 - 14:09

Contact: Guillaume Richer
Organisation: Vrije Universiteit Brussel
Status: Still in development, Published in peer reviewed journal
Follicle Biology Laboratory has developed a well characterized and standardized MOUSE in vitro follicle culture (IFC) system. In this system, early stage ovarian follicles are cultured in vitro under physiological hormone concentrations up to fertilizable and developmentally competent mature oocytes

Last updated on: 08-11-2019 - 14:00

Contact: Ellen Anckaert
Organisation: Vrije Universiteit Brussel
Status: History of use, Internally validated, Published in peer reviewed journal
In this method, it is possible to use active/viable animal or human brain slices / cells (normal or disease model) to study the effects of different drugs on brain cells (neuron or glia) in diverse brain region.

Last updated on: 08-11-2019 - 13:37

Contact: Surajit Sahu
Organisation: Vrije Universiteit Brussel
Status: History of use, Published in peer reviewed journal
Prototyping and replication (small series production) of microfluidic or optofluidic devices, in thermoplastic polymers or in glass. 3D nanoprinting is also available to produce microscaffolds, possibly within microfluidic channels.

Last updated on: 08-11-2019 - 13:36

Contact: Jürgen Van Erps
Organisation: Vrije Universiteit Brussel
Status: Internally validated, Published in peer reviewed journal
Recent improvements on the structural aspects of organ-on-chips pave the way towards a large-scale application. As such soon the number of read-out instruments that are in operation in parallel will need to drastically increase. Unfortunately, standard read-out equipment is bulky, complex and

Last updated on: 08-11-2019 - 13:33

Organisation: Vrije Universiteit Brussel
Status: Still in development
HepG2 is a human hepatoma derived cell line, which are epithelial in morphology. It was established from liver tissue of a 15-year-old Caucasian male with a well differentiated hepatocellular carcinoma. The HepG2 cell line is one of the most used human liver-based in vitro models. The cells secrete

Last updated on: 08-11-2019 - 10:11

Contact: Anja Heymans
Organisation: Vrije Universiteit Brussel
Status: History of use
Human umbilical cord-derived mesenchymal stromal cells (hUC-MSCs) express several key liver-specific transcription factors as well as hepatic progenitor markers. However, they still lack the hepatocyte nuclear factors 1-alpha (HNF1a) and 4-alpha (HNF4a), indispensable for their reprogramming towards

Last updated on: 08-11-2019 - 10:09

Contact: Karolien Buyl
Organisation: Vrije Universiteit Brussel
Status: Still in development
Albumin secretion in the supernatant of in vitro cultures can be measured by an enzyme-linked immunosorbent assay. The assay is based upon a quantitative sandwich enzyme immunoassay technique and measures albumin in the culture medium of primary hepatocytes and/or hepatocyte like cells. Standards

Last updated on: 08-11-2019 - 09:52

Contact: Steven Branson
Organisation: Vrije Universiteit Brussel
Status: History of use
Liver functionality can be monitored by the urea synthesis. In culture medium the measurement of urea synthesis relies on a chromogenic reagent that specifically forms a colored complex with urea. The concentration of this complex between urea, o-phthalaldehyde and N-(1-naphthyl) ethylenediamine can

Last updated on: 08-11-2019 - 09:49

Contact: Steven Branson
Organisation: Vrije Universiteit Brussel
Status: History of use
This method uses human skin-derived precursors (hSKP) differentiated towards hepatic cells (hSKP-HPC) as a hepatic in vitro model. Exposure of these cells for 24 hours to sub-cytotoxic concentrations of acetaminophen, which is a reference hepatotoxicant, induced specific cellular responses in a

Last updated on: 08-11-2019 - 09:48

Contact: Robim Rodrigues
Organisation: Vrije Universiteit Brussel
Status: Published in peer reviewed journal