Hemolysis assay to predict the inflammatory activity of inhaled particles

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Scope of the method

The Method relates to

Human health

The Method is situated in

Basic Research

Type of method

In vitro - Ex vivo

This method makes use of

Human derived cells / tissues / organs

Specify the type of cells/tissues/organs

human red blood cells

Description

Method keywords

membranolysis
red blood cells
erythrocytes
absorbance

Scientific area keywords

inflammation
inhaled particles
lung toxicity
silica

Method description

The hemolysis assay remarkably predicts the inflammatory potential of inhaled particles. The capacity of particles to damage cellular membranes is a key property to predict their inflammatory potential upon inhalation. In macrophages and epithelial cells exposed to particles, alteration of the phagolysosome membrane is a key event for the activation of the inflammasome and the release of interleukin-1beta. The membranolytic activity of particles can easily be assessed after incubation with red blood cells and measurement of the level of hemoglobin release. This assay can be performed with human red blood cells.

Lab equipment

Spectrophotometer.

Method status