Real-time bioenergetics in human and animal cell lines

Commonly used acronym: respirometry

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Scope of the method

The Method relates to
  • Environment
  • Human health
The Method is situated in
  • Basic Research
Type of method
  • In vitro - Ex vivo
This method makes use of
  • Animal derived cells / tissues / organs
Species from which cells/tissues/organs are derived
Human-derived cell lines
Type of cells/tissues/organs
Intestine, liver, lung, immune system

Description

Method keywords
  • respirometry
  • bioenergetics
  • Metabolism
  • mitochondrial respiration
  • glycolysis
Scientific area keywords
  • metabolism
  • toxicity
  • bioenergetics
  • Drug metabolism
Method description

Using respirometry (oxygen and pH, XF96 Analyzer), computer assisted cell analysis, and specific substrates and stressors, mitochondrial function and metabolic changes in a diverse set of cell lines can be measured. Relevant to study substrate preferences, acute and chronic effects of toxic substrates and contaminants, particles, as well as early events in the development of chronic diseases such as cancer and metabolic syndrome.

Lab equipment

XF96 Analyzer Agilent

Method status
  • History of use
  • Published in peer reviewed journal

Pros, cons & Future potential

Advantages

On-line measurement on real cell systems early responses chronic responses strong support by the company.

Challenges
  • - Variability;
  • - One provider relatively expensive consumables.
Modifications

Optimisation for tissues instead of cells.

Future & Other applications

Can be used in metabolic, cancer, toxicology and bioactive compounds research.

References, associated documents and other information

References

Marlies Decleer, Jelena Jovanovic, Anita Vakula, Bozidar Udovicki,, Rock-Seth E. K. Agoua, Annemieke Madder, Sarah De Saeger, and Andreja Rajkovic. Oxygen Consumption Rate Analysis of Mitochondrial Dysfunction Caused by Bacillus cereus Cereulide in Caco-2 and HepG2 Cells. 2018, Toxins, 10, 266.

Contact person

Charlotte Grootaert

Organisations

Ghent University (UGent)
Food Technology, Safety and Health
Belgium
Flemish Region