Culturing HEK 293 FT cells
Scope of the method
The Method relates to
- Human health
The Method is situated in
- Basic Research
- Translational - Applied Research
Type of method
- In vitro - Ex vivo
This method makes use of
- Human derived cells / tissues / organs
Specify the type of cells/tissues/organs
Human embryonic kidney 293 FT cells
Description
Method keywords
- Culturing
- Transfection
- Viral production
- High viral titer
Scientific area keywords
- Viral production
- High viral titer
- Clinical translation
- Cellular reprogramming
Method description
Human embryonic kidney (HEK) 293 FT cells is a celline that is very easy to culture and is used to obtain high viral titers. “293” is a reference to the 293th experiment wherein the cell line was discovered. A transfection with an adenovirus type 5 DNA fragment took place, causing the cell line to express E1A adenoviral gene. This stimulates the transcription of specific viral genes, resulting in a high production of viral proteins. “T” means that the HEK293 cell line is transfected with the SV40 T antigen, also stimulating the production of viral proteins. “F” stands for a fast growing HEK 293T strain with a high transfection efficiency.
Lab equipment
- Biosafety cabinet;
- Microscope;
- Incubator.
Method status
- History of use
Pros, cons & Future potential
Advantages
- High viral titer;
- Easy to culture;
- Fast growing;
- Easy to transfect.
Challenges
Use of serum.
Contact person
Matthias RombautOrganisations
Vrije Universiteit Brussel (VUB)Pharmaceutical and Pharmacological Sciences
In Vitro Toxicology and Dermato-Cosmetology
Belgium
Brussels Region