in vitro MDSC-T cell immunosuppression assay

Myeloid progenitor cells derived from the bone marrow of mice are stimulated using a cytokine mixture to become myeloid derived suppressor cell (MDSC) like cells. These are co-cultured with activated CD8+ T-cells derived from a mouse spleen. The MDSC like cells will suppress the proliferation of the T-cells in co-culture which can be observed by staining the CD8+ T-cells using a proliferation dye and flow cytometry. This assay can then be used to screen immunotherapeutic compounds on their capacity to reduce the immunosuppressive effects of MDSC.