The large (1mm diameter), easily accessible eggs of the frog Xenopus laevis (100-1000 eggs at once per frog) offer the opportunity to reconstitute cell cycle events in-vitro by generating cell-free extracts which retain all the biochemical components that regulate cell cycle progression, as well as
Last updated on: 22-07-2025 - 09:40
Contact: Martina Boiardi
Organisation: Katholieke Universiteit Leuven (KUL), Cellular and Molecular Medicine - KU Leuven
Status: History of use, Published in peer reviewed journal
We developed a biventricular statistical shape model (SSM) from high-resolution cardiac CT scans from 271 healthy individuals. Leveraging the diversity captured by our biventricular SSM, we created a synthetic cohort of anatomically detailed, high-resolution, biventricular meshes. The geometries in
Last updated on: 16-07-2025 - 10:01
Contact: Lore Van Santvliet
Organisation: Katholieke Universiteit Leuven (KUL)
Status: Published in peer reviewed journal
The most commonly used readout for eye function in fruit flies is the electroretinogram (ERG). While ERGs are applied to study phototransduction, they also constitute a robust assay to assess neuronal communication between photoreceptors and second-order brain neurons. Using glass electrodes placed
Last updated on: 02-06-2025 - 10:09
Contact: Valerie Uytterhoeven
Organisation: VIB - KU Leuven, Neurosciences - KU Leuven, Katholieke Universiteit Leuven (KUL)
Status: Published in peer reviewed journal
Longitudinal imaging of bioluminescent fungi in Galleria mellonella can complement standard survival and health scoring data by quantifying the fungal burden over time and monitoring early infection before clinical symptoms arise, and by more sensitively and more early detection of treatment effects
Last updated on: 28-05-2025 - 13:51
Contact: Eliane Vanhoffelen
Organisation: Katholieke Universiteit Leuven (KUL)
Status: Internally validated, Published in peer reviewed journal
Identifying drug-target interactions is a crucial step in drug repositioning, the process of suggesting new indications for known drugs. There are about 9000 FDA-approved and experimental small molecule drugs and more than 500.000 protein records available. Performing in vitro experiments would be
Last updated on: 28-01-2025 - 16:41
Contact: Daniele Parisi
Organisation: Katholieke Universiteit Leuven (KUL)
Partners: Biotech/TU-Dresden, Max-Planck-Institut für Informatik Saarbrucken
Status: Still in development
The protocol is aimed at developing primary human breast organoids that have a morphology similar to the one observed in the in vivo breast. This morphology encompasses a complex network organization composed of interconnected branches that terminate in TDLU-like structures. The organoids are
Last updated on: 11-10-2024 - 15:19
Contact: Colinda Scheele
Organisation: Katholieke Universiteit Leuven (KUL), Oncology - KU Leuven
Status: Still in development, History of use, Published in peer reviewed journal
The mammary-specific polyomavirus middle T antigen overexpression mouse model (MMTV-PyMT) is one of the most commonly used models in the cancer research field for multiple reasons, among which the spontaneous development of multifocal luminal tumors, the early tumoral onset, and the primary tumors'
Last updated on: 11-10-2024 - 15:19
Contact: Colinda Scheele
Organisation: Oncology - KU Leuven, Katholieke Universiteit Leuven (KUL)
The use of three-dimensional (3D) cell culture models is considered a reliable model that is successfully used for high-throughput drug testing in carcinoma research. However, the development of soft tissue sarcoma organoids is lagging behind. Soft tissue sarcoma is a group of rare malignancies from
Last updated on: 30-07-2024 - 12:08
Contact: Agnieszka Wozniak
Organisation: Oncology - KU Leuven, Katholieke Universiteit Leuven (KUL)
Status: Still in development
Using the combination of advanced CRIPSR tools including several Cas orthologs, based editors (ABE, CBE, CGBE) and prime editing technologie with efficient delivery vehicles such as LV, AAV and virus-like particles (VLPs), our lab has become experienced with introducing or replacing precise edits in
Last updated on: 21-06-2024 - 11:29
Contact: Marianne Carlon
Organisation: Katholieke Universiteit Leuven (KUL)
Partners: Katholieke Universiteit Leuven (KUL)
Status: Still in development, Internally validated
The DETECTOR algorithm allows to functionally screen genetic strategies for Cystic Fibrosis in patient-derived rectal organoids. DETECTOR is a machine-learning based software that takes frames from forskolin-induced swelling (FIS) assay on organoids as input and gives the number of functionally
Last updated on: 10-06-2024 - 11:17
Contact: Marianne Carlon
Organisation: Katholieke Universiteit Leuven (KUL)
Status: Published in peer reviewed journal
