Ibidi flow system immune cell adhesion assay
Commonly used acronym: Adhesion assay
Scope of the method
The Method relates to
- Animal health
- Human health
The Method is situated in
- Basic Research
- Translational - Applied Research
Type of method
- In vitro - Ex vivo
This method makes use of
- Animal derived cells / tissues / organs
Description
Method keywords
- adhesion
- endothelial cells
- migration
- shear stress
- blood brain barrier
- blood flow
Scientific area keywords
- immune cell migration
- human white blood cells
- blood brain barrier
- multiple sclerosis
- cell adhesion
- T lymphocyte
- blood vessel
Method description
Using an in vitro flow system adhesion assay, immune cell adhesion to (blood-brain barrier) endothelial cells can be quantified under physiological blood flow conditions. Endothelial cells can be treated with inflammatory cytokines or therapeutic antibodies to mimic inflammatory diseases. Immune cell adhesion (including rolling, probing or crawling) can be visualized using live-cell imaging.
Lab equipment
Ibidi pump system and slides to mimic physiological blood flow
Method status
- Published in peer reviewed journal
Pros, cons & Future potential
Advantages
- - Detailed analysis of migration phenotype (rolling, crawling, probing)
- - Compare different genotypes or treatment conditions
Challenges
- - Optimization required
- - Time consuming data acquisition and analysis
References, associated documents and other information
Links
Oncostatin M triggers brain inflammation by compromising blood-brain barrier in…
Contact person
Doryssa HermansOrganisations
University of Hasselt (UHasselt)Department of Immunology and Infection
Belgium
Flemish Region
