The scope of our research ranges from the study of flow and transport processes in blood and biological fluids in the cardiovascular system and artificial organs to biomechanical aspects of the cardiovascular and the skeleto-muscular system and medical devices. All research tracks explored by our

Last updated on: 12-07-2019 - 16:55

Contact: Patrick Segers
Organisation: Ghent University

The Koken rat is a dummy rat that is developed to train animal procedures, such as handling, blood sampling and intubation.

Last updated on: 10-07-2019 - 10:56

Contact: Cannot be disclosed
Organisation: Cannot be disclosed

This method describes the steps from a living rat to a single cell solution of primary hepatocytes. This requires surgery on the lab animal, a perfusion with buffer solution, a digestion with collagenase and a filtration step to obtain primary hepatocytes.

Last updated on: 08-05-2019 - 14:33

Contact: Paul Claes
Organisation: Vrije Universiteit Brussel

This method describes the steps to go from a liver to a decellularized matrix. It uses mild and strong detergents to destroy cells and keep the extracellular matrix intact. This matrix can then be used for a variety of purposes, including (but not limited to) repopulation, basis for coating and

Last updated on: 08-05-2019 - 14:33

Contact: Paul Claes
Organisation: Vrije Universiteit Brussel
Status: Published in peer reviewed journal

Rat liver epithelial cells (rLEC) can be isolated from 8-day old male Sprague-Dawley rats. Briefly, small fragments of neonatal rat livers are incubated for 15 minutes with 4-(2-hydroxyethyl)-1-piperazine-ethanesulfonic acid (HEPES) buffered trypsin solution [0.25 % (v/v)] and washed twice with

Last updated on: 08-05-2019 - 14:33

Contact: Joery De Kock
Organisation: Vrije Universiteit Brussel
Status: History of use, Internally validated, Published in peer reviewed journal

The DPRA is an in chemico method which quantifies the remaining concentration of cysteine- or lysine-containing peptide following 24 hours incubation with the test chemical at 252.5ºC. The synthetic peptides contain phenylalanine to aid in the detection. Relative peptide concentration

Last updated on: 17-04-2019 - 13:23

Contact: bart Desmedt
Organisation: Sciensano, Sciensano
Partners: Sciensano
Status: History of use, Internally validated, Validated by an external party (e.g. OECD, EURL ECVAM,…)

This method describes a well-known optimised human in vitro model of drug-induced cholestasis. Cryopreserved primary human hepatocytes are cultured between two layers of extracellular matrix scaffold, which will delay dedifferentiation and allows to restore cell-extracellular matrix interactions.

Last updated on: 02-04-2019 - 10:45

Contact: Eva Gijbels
Organisation: Vrije Universiteit Brussel
Status: Still in development

The monocyte-activation test (MAT) is used to detect or quantify substances that activate human monocytes or monocytic cells to release endogenous mediators such as pro-inflammatory cytokines, for example tumour necrosis factor alpha (TNFα), interleukin-1 beta (IL-1β) and interleukin-6 (IL-6).

Last updated on: 27-03-2019 - 16:50

Contact: Celine Vanhee
Organisation: Sciensano
Status: Still in development, History of use

This method uses human skin-derived precursors (hSKP) differentiated towards hepatic cells (hSKP-HPC) as an hepatic in vitro model. Exposure of these cells for 24 hours to sub-cytotoxic concentrations of acetaminophen, which is a reference hepatotoxicant, induced specific cellular responses in a

Last updated on: 27-03-2019 - 16:43

Contact: Robim Rodrigues
Organisation: Vrije Universiteit Brussel
Status: Published in peer reviewed journal