Intestinal organoids are cultured from intestinal biopsies obtained during routine endoscopy. The stem cell containing crypts are isolated and cultured in a 3D ECM (Matrigel) in the presence of the desired growth factors. The present stem cells will expand and give rise to all epithelial cells of

Last updated on: 15-10-2020 - 14:32

Contact: Cannot be disclosed
Organisation: KU Leuven
Status: Published in peer reviewed journal
Alkaline comet assay (COM) and Micronucleus test (MN) are well-established cytogenetic tests that are used to detect both immediate damages i.e. DNA fragmentation, and permanent damage which results in micronuclei formation. These techniques are often applied separately or, sometimes, in combination

Last updated on: 08-10-2020 - 10:59

Contact: Ha Nguyen
Organisation: Sciensano, Université libre de Bruxelles
Partners: Liège Université
Status: History of use, Internally validated
The CALUX method is an in vitro bioassay that uses reporter gene cell lines that have been stably transfected with a luciferase reporter gene under the control of relevant receptor specific DNA response element. This enables the screening for chemicals that can bind to specific receptors and

Last updated on: 17-09-2020 - 11:49

Contact: Imke Boonen
Organisation: Vrije Universiteit Brussel
Metabolomics and 13C tracer analysis are state-of-the-art techniques that allow determining the concentration of metabolites and the activity of metabolic pathways, respectively. Three dimensional (3D) cultures of cancer cells constitute an enriched in vitro environment that can be used to assay

Last updated on: 27-08-2020 - 14:42

Contact: Dorien Broekaert
Organisation: VIB KU Leuven, KU Leuven
Status: Published in peer reviewed journal
Isolation Femoral and tibial bones are isolated from 10-12 weeks old C57BL/6J mice. After cutting the bone's ends, the bone marrow is flushed out using a 23G syringe with PBS into a 50ml falcon tube. After centrifugation cells are re-suspended in FCS (0.5ml/mouse leg). Differentiation 0.5ml of the

Last updated on: 14-08-2020 - 15:26

Contact: Jerome Hendriks
Organisation: Hasselt University
Status: History of use, Internally validated, Published in peer reviewed journal
Characterization of Human Oligodendroglia derived-EV-rich in sHSPs (HOG-EV-sHSPs). Establish HSPB1/B8 stably expressing cell lines for the production of sHSPs rich-EV cell culture conditioned medium. Human Oligodendroglia cell lines (HOG) were stably transduced with pLenti-HSPBs gene constructs

Last updated on: 14-08-2020 - 15:22

Contact: Joy Irobi
Organisation: Hasselt University
Status: Still in development, Internally validated, Currently submitted for further validation by an external party (e.g. OECD, EURL ECVAM,…)
The feed undergoes an in vitro enzymatic hydrolysis simulating the digestion in the upper digestive tract followed by a dialysis mimicking the intestinal absorption. Finally, an in vitro batch fermentation is performed and simulates the fermentation occurring in the hindgut. The ingredients, ground

Last updated on: 16-07-2020 - 15:04

Contact: Nadia Everaert
Organisation: Liège Université
Status: Published in peer reviewed journal
Prebiotics are used by the gut microbiota creating metabolites that exert a positive effect on the host (postbiotics). Prebiotics are available on the market for human and animal consumption and may induce differential effects on the host's health and metabolism. They can be tested by in vitro

Last updated on: 16-07-2020 - 15:02

Contact: Nadia Everaert
Organisation: Liège Université
Status: Published in peer reviewed journal
Cardiomyocytes derived from induced pluripotent stem cells (iPSC-CMs) offer an attractive platform for cardiovascular research, including disease modeling, drug toxicity testing and development of regenerative therapies. Patient-specific iPSC-CMs are very useful to study disease pathogenesis and

Last updated on: 30-06-2020 - 14:13

Contact: Maaike Alaerts
Organisation: University of Antwerp
Status: Still in development, Internally validated
Vascular smooth muscle cell (VSMC) deficiency plays a pivotal role in aneurysm development. Unfortunately, access to native VSMCs of patients and (particularly) control individuals is extremely limited. It has been shown that iPSC-derived VSMCs recapitulate the yet known disease processes very well.

Last updated on: 03-06-2020 - 13:51

Organisation: University of Antwerp
Status: Still in development, Internally validated