This method describes the steps from a living rat to a single cell solution of primary hepatocytes. This requires surgery on the lab animal, a perfusion with buffer solution, a digestion with collagenase and a filtration step to obtain primary hepatocytes.

Last updated on: 08-05-2019 - 14:33

Contact: Paul Claes
Organisation: Vrije Universiteit Brussel

Rat liver epithelial cells (rLEC) can be isolated from 8-day old male Sprague-Dawley rats. Briefly, small fragments of neonatal rat livers are incubated for 15 minutes with 4-(2-hydroxyethyl)-1-piperazine-ethanesulfonic acid (HEPES) buffered trypsin solution [0.25 % (v/v)] and washed twice with

Last updated on: 08-05-2019 - 14:33

Contact: Joery De Kock
Organisation: Vrije Universiteit Brussel
Status: History of use, Internally validated, Published in peer reviewed journal

This method describes the steps to go from a liver to a decellularized matrix. It uses mild and strong detergents to destroy cells and keep the extracellular matrix intact. This matrix can then be used for a variety of purposes, including (but not limited to) repopulation, basis for coating and

Last updated on: 08-05-2019 - 14:33

Contact: Paul Claes
Organisation: Vrije Universiteit Brussel
Status: Published in peer reviewed journal

The DPRA is an in chemico method which quantifies the remaining concentration of cysteine- or lysine-containing peptide following 24 hours incubation with the test chemical at 252.5ºC. The synthetic peptides contain phenylalanine to aid in the detection. Relative peptide concentration

Last updated on: 17-04-2019 - 13:23

Contact: bart Desmedt
Organisation: Sciensano, Sciensano
Partners: Sciensano
Status: History of use, Internally validated, Validated by an external party (e.g. OECD, EURL ECVAM,…)

This method describes a well-known optimised human in vitro model of drug-induced cholestasis. Cryopreserved primary human hepatocytes are cultured between two layers of extracellular matrix scaffold, which will delay dedifferentiation and allows to restore cell-extracellular matrix interactions.

Last updated on: 02-04-2019 - 10:45

Contact: Eva Gijbels
Organisation: Vrije Universiteit Brussel
Status: Still in development

The monocyte-activation test (MAT) is used to detect or quantify substances that activate human monocytes or monocytic cells to release endogenous mediators such as pro-inflammatory cytokines, for example tumour necrosis factor alpha (TNFα), interleukin-1 beta (IL-1β) and interleukin-6 (IL-6).

Last updated on: 27-03-2019 - 16:50

Contact: Celine Vanhee
Organisation: Sciensano
Status: Still in development, History of use

This method uses human skin-derived precursors (hSKP) differentiated towards hepatic cells (hSKP-HPC) as an hepatic in vitro model. Exposure of these cells for 24 hours to sub-cytotoxic concentrations of acetaminophen, which is a reference hepatotoxicant, induced specific cellular responses in a

Last updated on: 27-03-2019 - 16:43

Contact: Robim Rodrigues
Organisation: Vrije Universiteit Brussel
Status: Published in peer reviewed journal

HeLa cells are the first continuous cancer cell line and were isolated from the aggressive glandular cervical cancer of a 31-year old woman. It was the first aneuploid line derived from human tissue maintained in continuous cell culture. Knowledge of almost every process that occurs in human

Last updated on: 22-03-2019 - 11:39

Organisation: Vrije Universiteit Brussel
Status: Still in development, History of use