We developed a transgenic zebrafish line that expresses a genetically encoded calcium and voltage indicator in the heart, allowing us to assess cardiac action potentials and calcium transients in vivo at real time by analysing changes in fluorescent signal.
Last updated on: 08-05-2025 - 17:38
Contact: Dorien Schepers
Organisation: University of Antwerp (UAntwerpen)
Status: Internally validated
This method uses human pluripotent stem cells (hPSCs), including embryonic and induced pluripotent stem cells, to generate cortical brain organoids and model brain tumorigenesis through targeted genetic manipulation. The aim is to create a physiologically relevant, in vitro 3D system that mimics
Last updated on: 06-05-2025 - 15:34
Contact: Diana Al Delbany
Organisation: Vrije Universiteit Brussel (VUB)
Status: History of use, Internally validated, Published in peer reviewed journal
Cholestatic drug-induced liver injury (cDILI) is a frequent reason for drug failure and withdrawal during premarketing and postmarketing stages of drug development. Strategies for reliable detection of cDILI in early drug development are therefore urgently needed. The drug-induced cholestasis index
Last updated on: 23-04-2025 - 11:36
Contact: Cannot be disclosed
Organisation: Vrije Universiteit Brussel (VUB)
Status: Published in peer reviewed journal
The Colon-on-a-plate® technology is a high-throughput biorelevant in vitro simulation of the physiology and microbiology of the colon. This robust screening technology is not limited to comparing the impact of tens of test product on the microbiome, but also offers insight into the factors
Last updated on: 11-04-2025 - 16:13
Contact: Cannot be disclosed
Organisation: ProDigest
Status: History of use, Internally validated, Published in peer reviewed journal
The M-SHIME is a dynamic model for the human digestive tract that mimics the different compartments - stomach, small intestine and colon - while also incorporating a cross-sectional simulation mimicking both the luminal as the mucosal microenvironment of the human gut. The mucosal environment in
Last updated on: 04-03-2025 - 13:23
Contact: Tom Van de Wiele
Organisation: Ghent University (UGent)
Status: History of use, Internally validated, Published in peer reviewed journal
Pharmaceutical products intended for parenteral use must be free from pyrogenic (fever–inducing) contamination. Pyrogens comprise endotoxin from Gram-negative bacteria and non-endotoxin pyrogens (NEP) from Gram-positive bacteria, viruses, and fungi. The longstanding compendial test for pyrogens is
Last updated on: 29-01-2025 - 10:44
Contact: Philip Breugelmans
Organisation: Janssen Pharma of JNJ
Partners: Janssen Pharma of JNJ
Status: Internally validated, Published in peer reviewed journal
The BALB/c-derived - 4T1 mammary tumor cell line and RAW264.7 macrophage cell line were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% heat-inactivated fetal bovine serum (FBS), 100 U/ml penicillin and 100 μg/ml streptomycin in culture flasks. Harvesting of cultured
Last updated on: 28-01-2025 - 16:41
Contact: Kristel Demeyere
Organisation: Ghent University (UGent)
Status: Published in peer reviewed journal
Identifying drug-target interactions is a crucial step in drug repositioning, the process of suggesting new indications for known drugs. There are about 9000 FDA-approved and experimental small molecule drugs and more than 500.000 protein records available. Performing in vitro experiments would be
Last updated on: 28-01-2025 - 16:41
Contact: Daniele Parisi
Organisation: Katholieke Universiteit Leuven (KUL)
Partners: Biotech/TU-Dresden, Max-Planck-Institut für Informatik Saarbrucken
Status: Still in development
Human embryonic kidney (HEK) 293 FT cells is a celline that is very easy to culture and is used to obtain high viral titers. “293” is a reference to the 293th experiment wherein the cell line was discovered. A transfection with an adenovirus type 5 DNA fragment took place, causing the cell line to
Last updated on: 28-01-2025 - 15:12
The Chorioallantoic Membrane (CAM) assay is a versatile, cost-effective in ovo model using the vascular-rich membrane of fertilized chicken eggs to study biological processes such as angiogenesis, tumor growth, metastasis, and drug testing. Its transparency and rapid vascularization make it ideal
Last updated on: 19-12-2024 - 11:45
Contact: Lotte Alders
Organisation: University of Hasselt (UHasselt)
Status: History of use, Published in peer reviewed journal
