Advances in three-dimensional (3D) culture techniques have shown several advantages over 2D cultures, especially by more accurately mimicking the in vivo environment. This has led to improved reproducibility and reliability of experimental results, which are important criteria in disease modelling

Last updated on: 14-10-2021 - 16:59

Organisation: Ghent University
Status: Still in development, Published in peer reviewed journal
The cardiorespiratory model reproduces human physiology with a high level of fidelity. It is a lumped parameter model including a representation of atria, ventricles, pulmonary and systemic circulations, autonomic controls, metabolic peripheral control, ventilation and gas exchange in tissues and

Last updated on: 13-10-2021 - 10:47

Contact: Libera Fresiello
Organisation: Cannot be disclosed
Partners: Cannot be disclosed
Status: History of use, Internally validated, Published in peer reviewed journal
In the skillslab, dummy models and simulators are used for teaching various clinical skills such as sutures, injections, … The veterinarians in training start with practising basic skills such as handling animals, and progress over the years to perform more complex procedures on simulators such as

Last updated on: 08-10-2021 - 16:17

Organisation: Ghent University (UGent)
Status: History of use, Internally validated
We aimed to optimize fibrin matrix composition in order to mimic human ovarian tissue architecture for human ovarian follicle encapsulation and grafting. Ultrastructure of fresh human ovarian cortex in age-related women (n = 3) and different fibrin formulations (F12.5/T1, F30/T50, F50/T50, F75/T75),

Last updated on: 10-09-2021 - 14:18

Organisation: UCLouvain
Status: Published in peer reviewed journal
Quantum Dots (QDs) have been hypothesized as potential probes for the optical monitoring of electrogenic cell activity given their high optical absorption cross-sections compared to organic fluorophores, high brightness and low photobleaching. Despite the theoretical predictions, less than a handful

Last updated on: 04-08-2021 - 16:50

Contact: Stijn Jooken
Organisation: Katholieke Universiteit Leuven (KUL)
Status: Published in peer reviewed journal
The response of sublingual epithelial cells to house dust mite allergen and potential tolerance-promoting adjuvants such as Toll-like receptor (TLR) ligands and calcitriol was investigated using primary sublingual epithelial cells isolated from dogs and cultured in vitro. After 24-h incubation with

Last updated on: 07-04-2021 - 14:52

Contact: Eric Cox
Organisation: Ghent University
Status: Published in peer reviewed journal
The method is described in detail in OECD TG 439 as SkinEthicTM RHE and provides an in vitro procedure that may be used for the hazard identification of irritant chemicals (substances and mixtures) in accordance with the United Nations Globally Harmonized System of Classification and Labelling (UN

Last updated on: 11-03-2021 - 19:17

Organisation: Vlaamse Instelling voor Technologisch Onderzoek (VITO)
Status: Validated by an external party (e.g. OECD, EURL ECVAM,…)
We used a 7-stage protocol to generate beta cells from human Induced Pluripotent Stem Cells (iPSC) and evaluated whether these cells are responsive to the pro-inflammatory cytokines (IFNγ, IL-1β, or IFNα) that play a role in type 1 diabetes (T1D). Our data show that human iPSC-derived beta cells

Last updated on: 09-03-2021 - 21:30

Contact: Decio L. Eizirik
Organisation: Université libre de Bruxelles, Indiana Biosciences Research Institute
Status: Published in peer reviewed journal
The ERα CALUX transactivation assay is described in Annex 4 of the OECD TG 455. It uses the human U2OS cell line to detect estrogenic agonist and antagonist activity mediated through human estrogen receptor alpha (hERα).This test method is specifically designed to detect hERα-mediated

Last updated on: 04-03-2021 - 16:33

Contact: Cannot be disclosed
Organisation: VITO
Status: Validated by an external party (e.g. OECD, EURL ECVAM,…)
The method has been fully described in OECD TG 236 and is intended to determine the acute or lethal toxicity of chemicals on embryonic stages of fish (Danio rerio). Newly fertilized zebrafish eggs are exposed to the test chemical for a period of 96 hrs. Every 24 hrs. Twenty embryos (one embryo per

Last updated on: 04-03-2021 - 16:29

Contact: Hilda Witters
Organisation: Vlaamse Instelling voor Technologisch Onderzoek (VITO)
Status: Published in peer reviewed journal, Validated by an external party (e.g. OECD, EURL ECVAM,…)