The present standard procedure describes a protocol for measuring the urea concentration in supernatant of human stem cell-derived hepatocyte-like cells. This procedure relies on a chromogenic reagent that forms a colored complex specifically with urea. The latter can be measured and is directly

Last updated on: 22-03-2019 - 11:30

Contact: Cannot be disclosed
Organisation: Vrije Universiteit Brussel
Status: History of use, Internally validated

Basically, the standard operating procedure outlined in this document consists of the following steps, namely, (i) preparation of the cells for the DCFH-DA assay, (ii) DCFH-DA test procedure, (v) processing of the results. Practical details are provided for each of these steps and are followed by

Last updated on: 22-03-2019 - 11:30

Contact: Cannot be disclosed
Organisation: Vrije Universiteit Brussel
Status: History of use, Internally validated

This method describes a very reliable and robust in vitro model for the screening of the cholestatic liability of drugs and other chemical entities. The 3D spheroids generated from primary human hepatocytes can be cultivated up to 28 days, allowing long-term exposures which can depict otherwise

Last updated on: 22-03-2019 - 11:29

Organisation: Vrije Universiteit Brussel
Status: Still in development

The method detects two facets of drug-induced cytotoxicity i.e. the intracellular accumulation of phospholipids and of neutral lipids, i.e. phospholipidosis and steatosis respectively. The assay makes use of a kit containing an aqueous, red-fluorescent formulation of labelled phospholipids

Last updated on: 22-03-2019 - 11:29

Contact: Kaat Leroy
Organisation: Vrije Universiteit Brussel
Status: History of use

This method assesses general cytotoxicity. Upon disruption of the cell membrane, lactate dehydrogenase (LDH) is released. LDH catalyzes the interconversion of pyruvate and lactate with concomitant interconversion of reduced (NADH) and oxidized (NAD+) nicotinamide adenine dinucleotide. The

Last updated on: 22-03-2019 - 11:28

Contact: Kaat Leroy
Organisation: Vrije Universiteit Brussel
Status: History of use

Human embryonic kidney (HEK) 293 FT cells is a celline that is very easy to culture and is used to obtain high viral titers. “293” is a reference to the 293th experiment wherein the cell line was discovered. A transfection with an adenovirus type 5 DNA fragment took place, causing the cell line

Last updated on: 22-03-2019 - 11:28

Contact: Matthias Rombaut
Organisation: Vrije Universiteit Brussel
Status: History of use

The neutral red uptake assay is a cell viability assay that allows in vitro quantification of xenobiotic-induced cytotoxicity. The assay relies on the ability of living cells to incorporate and bind neutral red, a weak cationic dye, in lysosomes. As such, cytotoxicity is expressed as a

Last updated on: 22-03-2019 - 11:27

Contact: Robim Rodrigues
Organisation: Vrije Universiteit Brussel
Status: Published in peer reviewed journal, Validated by an external party (e.g. OECD, EURL ECVAM,…)

HepG2 is a human hepatoma derived cell line, which are epithelial in morphology. It was established from liver tissue of a 15-year-old Caucasian male with a well differentiated hepatocellular carcinoma. The HepG2 cell line is one of the most used human liver-based in vitro models. The cells

Last updated on: 22-03-2019 - 10:57

Contact: Anja Heymans
Organisation: Vrije Universiteit Brussel
Status: History of use

Cryopreserved differentiated HepaRG cells (obtained from Biopredic International) are derived from a human hepatocellular carcinoma. These cells are an unique in vitro tool that provides reproducible results and exhibit many characteristics of primary human hepatocytes such as morphology,

Last updated on: 21-03-2019 - 16:49

Contact: Anja Heymans
Organisation: Vrije Universiteit Brussel, Vrije Universiteit Brussel
Status: History of use

By the use of monolayer cultures as a in vitro system, the effects of drugs on CYP3A activity can be evaluated. It relies on the use of a luminogenic CYP3A substrate, namely luciferin-6′-pentafluorobenzylether (luciferin- PFBE). Upon biotransformation by CYP3A, luciferin-PFBE is converted into

Last updated on: 21-03-2019 - 09:34

Contact: Steven Branson
Organisation: Vrije Universiteit Brussel
Status: History of use