The protocol is aimed at developing primary human breast organoids that have a morphology similar to the one observed in the in vivo breast. This morphology encompasses a complex network organization composed of interconnected branches that terminate in TDLU-like structures. The organoids are
Last updated on: 07-11-2022 - 11:24
Contact: Beaunelle de Bruijn
Organisation: Katholieke Universiteit Leuven (KUL), Oncology - KU Leuven
Status: Still in development, History of use, Published in peer reviewed journal
These are the general guidelines on sample input requirements to successfully start our RIBO-seq procedures:
Cell lines in suspension: pellet of 10 – 50 M snap frozen cells. The pre-treatment and collection specifications are also mentioned in our pre-processing protocol.
Adherent cell lines
Last updated on: 21-10-2022 - 10:33
Contact: Zarel Martinez
Organisation: OHMX.bio
Status: History of use, Internally validated, Published in peer reviewed journal
After digestion with pronase, human epithelial cells are cultured on flask and then on inserts to recapitulate a fully differentiated epithelium.
Last updated on: 19-10-2022 - 16:54
Contact: Sophie Gohy
Organisation: Cliniques universitaires Saint-Luc, UCLouvain
Status: Published in peer reviewed journal
We have developed a protocol for culturing primary neuron progenitor cells (NPCs) derived from mouse embryos at embryonic day 15 (E15). These cells are highly proliferative, can be subcultured and cryopreserved and can be differentiated to neurons or astrocytes. Hence, this method can greatly reduce
Last updated on: 20-10-2022 - 08:55
Contact: Roel Quintens
Organisation: Belgian Nuclear Research Centre
Status: Still in development, History of use, Internally validated
The protocol is aimed at developing primary mammary gland organoids that have a morphology similar to the one of the in vivo breast, which is organized as a complex network of interconnected branches. The organoids are derived from the mouse mammary gland by mechanical dissociation and enzymatic
Last updated on: 07-11-2022 - 11:08
Contact: Beaunelle de Bruijn
Organisation: Oncology - KU Leuven
Status: History of use, Published in peer reviewed journal
This method is used to let cells interact for a better simulation of processes occurring in the body. The rational of the method is that monoculture experiments do not capture the complexity of in vivo interactions between different organs. In the most simple setup (published work Nutrients), Caco-2
Last updated on: 09-02-2023 - 16:37
Contact: Amar van Laar
Organisation: Ghent University (UGent)
Status: Published in peer reviewed journal
The mammary-specific polyomavirus middle T antigen overexpression mouse model (MMTV-PyMT) is one of the most commonly used models in the cancer research field for multiple reasons, among which the spontaneous development of multifocal luminal tumors, the early tumoral onset, and the primary tumors'
Last updated on: 07-11-2022 - 11:47
Contact: Beaunelle de Bruijn
Organisation: Oncology - KU Leuven, Katholieke Universiteit Leuven (KUL)
ZeptoWard is a Machine Learning solution (AI) which identifies the ADMET properties of compounds. It can accurately predict over 80 properties related to absorption, distribution, metabolism, excretion, and toxicity properties, how a specific compound or combination of compounds will perform. It can
Last updated on: 21-03-2023 - 13:34
ZeptoHit is a technology based on Artificial Intelligence (AI) which accelerates hit discovery. With only a protein sequence as information, ZeptoHit can quickly and efficiently recommend promising hits, without any limitations. Its search for promising compounds goes much beyond chemical structures
Last updated on: 21-03-2023 - 13:36
Contact: Segolene Martin
Organisation: Kantify
Status: Internally validated, Published in peer reviewed journal
The tri-dimensional (3D) culture protocols allow isolation and culture of gastric epithelial antral and intestinal stem cells to efficiently generate organoids that recapitulate the mature pyloric and intestinal epithelium in vitro and foetal epithelial progenitors of these tissues growing as
Last updated on: 16-08-2022 - 10:57
Contact: Marie-Isabelle Garcia
Organisation: Université Libre de Bruxelles (ULB)
Status: History of use, Internally validated, Published in peer reviewed journal
