We established a protocol to efficiently generate hepatic stellate cells (HSCs) from human pluripotent stem cells (PSCs). Our procedure generated complex in vitro spheroid cultures that better mimic the complexity of the liver as well as liver function. In co-culture, iPSC-HSCs promote maintenance

Last updated on: 04-11-2020 - 12:32

Organisation: Vrije Universiteit Brussel
Partners: KU Leuven
Status: Published in peer reviewed journal
The CALUX method is an in vitro bioassay that uses reporter gene cell lines that have been stably transfected with a luciferase reporter gene under the control of relevant receptor specific DNA response element. This enables the screening for chemicals that can bind to specific receptors and

Last updated on: 17-09-2020 - 11:49

Contact: Imke Boonen
Organisation: Vrije Universiteit Brussel
Amoebae are natural eukaryotic predators of bacteria, yeasts, fungi and they are ubiquiste. They are excellent and easy-to-use cellular infection models, as they allow to co-cultivate any organisms in a broad range of infection medium, compatible with high quality microscopy techniques,

Last updated on: 19-05-2020 - 11:02

Organisation: Vrije Universiteit Brussel
Status: Internally validated, Published in peer reviewed journal
A calculated number of bacteria is injected through the pro-legs of the larvae. As several entry points are present, this allows to inject virulent bacteria and potential antimicrobial compounds within the same host. The survival of the larvae is scored over time (days post inoculation) until about

Last updated on: 19-05-2020 - 11:01

Organisation: Vrije Universiteit Brussel
Status: Internally validated, Published in peer reviewed journal
Follicle Biology Laboratory has developed a well characterized and standardized MOUSE in vitro follicle culture (IFC) system. In this system, early stage ovarian follicles are cultured in vitro under physiological hormone concentrations up to fertilizable and developmentally competent mature oocytes

Last updated on: 08-11-2019 - 14:00

Contact: Ellen Anckaert
Organisation: Vrije Universiteit Brussel
Status: History of use, Internally validated, Published in peer reviewed journal
Prototyping and replication (small series production) of microfluidic or optofluidic devices, in thermoplastic polymers or in glass. 3D nanoprinting is also available to produce microscaffolds, possibly within microfluidic channels.

Last updated on: 08-11-2019 - 13:36

Contact: Jürgen Van Erps
Organisation: Vrije Universiteit Brussel
Status: Internally validated, Published in peer reviewed journal
Recent improvements on the structural aspects of organ-on-chips pave the way towards a large-scale application. As such soon the number of read-out instruments that are in operation in parallel will need to drastically increase. Unfortunately, standard read-out equipment is bulky, complex and

Last updated on: 08-11-2019 - 13:33

Organisation: Vrije Universiteit Brussel
Status: Still in development
Using this method you can measure the (relative) lipid load in human skin-derived stem cells differentiated towards hepatic cells. This method could also be applied on other cell types (e.g. HepG2), since it is based on the following publication: "M. T. Donato et al., Chem. Biol. Interact. 181, 417

Last updated on: 08-11-2019 - 09:39

Contact: Joost Boeckmans
Organisation: Vrije Universiteit Brussel
Status: Internally validated
HeLa cells are the first continuous cancer cell line and were isolated from the aggressive glandular cervical cancer of a 31-year old woman. It was the first aneuploid line derived from human tissue maintained in continuous cell culture. Knowledge of almost every process that occurs in human cells

Last updated on: 08-11-2019 - 09:25

Organisation: Vrije Universiteit Brussel
Status: Still in development, History of use