Immersion treatment: zebrafish embryos at the two-cell stage were immersed in Danieau’s medium containing the compound. A compound concentration of 10 µM and DMSO concentration of 0.1% (v/v) in a volume of 5 mL was used per well (6-well-plates). At 72 h, the zebrafish medium containing the compound

Last updated on: 01-09-2023 - 09:32

Contact: Marie Pardon
Organisation: Katholieke Universiteit Leuven (KUL)
Status: Published in peer reviewed journal
The method is an approach to systematically study the impact of the nutrient microenvironment on the metabolism of effector CD8+ T cells, based on performing stable 13C isotope labeling measurements on in vitro-differentiated murine effector CD8+ T cells. Naive CD8+ T cells are isolated from mouse

Last updated on: 31-08-2023 - 17:12

Contact: Dorien Broekaert
Organisation: Katholieke Universiteit Leuven (KUL), VIB - KU Leuven
Status: Published in peer reviewed journal
Metabolomics and 13C tracer analysis are state-of-the-art techniques that allow determining the concentration of metabolites and the activity of metabolic pathways, respectively. Three dimensional (3D) cultures of cancer cells constitute an enriched in vitro environment that can be used to assay

Last updated on: 31-08-2023 - 17:12

Contact: Dorien Broekaert
Organisation: VIB - KU Leuven, Katholieke Universiteit Leuven (KUL)
Status: Published in peer reviewed journal
This assay evaluates the sprouting ability of endothelial cells in a collagen matrix and it used as an in vitro model to study the formation of new blood/lymphatic vessels. The effect of pro-angiogenic growth factors or co-cultured cells can be measured by quantifying the amount of vascular sprouts

Last updated on: 25-08-2023 - 09:11

Contact: Diede Houbaert
Organisation: Cellular and Molecular Medicine - KU Leuven
Status: History of use, Internally validated, Published in peer reviewed journal
NMTox is an R-software package and a Shiny app that can be used to explore and subset large datasets and can identify and test for monotonic dose responses. The package was developed within the NanoInformaTIX project where a platform is developed that aims to predict nanomaterial toxicity.

Last updated on: 22-08-2023 - 10:43

Contact: Geert Verheyen
Organisation: Thomas More University of Applied Sciences, University of Hasselt (UHasselt)
Status: Internally validated
The use of patient-derived primary cell cultures in cancer preclinical assays, including drug screens and genotoxic studies, has increased in recent years. However, their translational value is constrained by several limitations, including variability that can be caused by the culture conditions.

Last updated on: 20-07-2023 - 15:05

Contact: Ahmad Najem
Organisation: Institut Jules Bordet
Status: Published in peer reviewed journal
In the skillslab, dummy models and simulators are used for teaching various clinical skills. The veterinarians in training need to learn how to assist the calving process in the cow and how to perform artifical insemination and rectal palpation in cows. Although training on living animals is

Last updated on: 28-06-2023 - 13:53

Organisation: Ghent University (UGent)
Status: Still in development, History of use, Internally validated
In the skillslab, dummy models and simulators are used for teaching various clinical skills. The veterinarians in training need to learn how to handle cows and pigs in a correct and safe way. Although training on living animals is indispensable, a part of the training process can be performed on

Last updated on: 28-06-2023 - 13:53

Organisation: Ghent University (UGent)
Status: History of use, Internally validated
In the skillslab, dummy models and simulators are used for teaching various clinical skills. The veterinarians in training need to learn how to monitor and approach an animal under anesthesia. Furthermore, they need to learn ventilation techniques and comprehension of an anesthetic machine. An

Last updated on: 23-06-2023 - 17:14

Organisation: Ghent University (UGent)
Status: History of use, Internally validated
In the skillslab, dummy models and simulators are used for teaching various clinical skills. Endotracheal intubation after induction of anesthesia is an important and commonly performed procedure in veterinary practice. An important part of this training process can be performed on a simulator in

Last updated on: 23-06-2023 - 17:13

Organisation: Ghent University (UGent)
Status: History of use, Internally validated