3D human and/or animal ex vivo precision cut tissue slices

Ex-vivo tissue explants (precision cut tissue slices) prepared with the Krumdieck Tissue Slicer are living, three-dimensional tissue slices closely resemble the organ from which it is prepared, with all the cell types present in their original tissue-matrix configuration where physiological and

Last updated on: 31-01-2023 - 15:52

Contact: Bella Manshian
Organisation: Katholieke Universiteit Leuven (KUL)
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Human Intestinal Enteroids as a model for viral infection

Human Intestinal Organoids (HIOs) are in vitro 3D cell cultures arranged in a crypt-villus structure that incorporate many physiological features of the intestinal epithelium, including the presence of different cell populations (enterocytes, goblet cells, enteroendocrine and Paneth cells). HIOs can

Last updated on: 27-01-2023 - 13:29

Contact: Joana Rocha-Pereira
Organisation: Katholieke Universiteit Leuven (KUL)
Status: Published in peer reviewed journal
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3D organoids and organoid derived monolayers from patients with inflammatory bowel disease

Intestinal organoids are cultured from intestinal biopsies obtained during routine endoscopy. The stem cell containing crypts are isolated and cultured in a 3D ECM (Matrigel) in the presence of the desired growth factors. The present stem cells will expand and give rise to all epithelial cells of

Last updated on: 11-01-2023 - 16:43

Contact: Bram Verstockt
Organisation: Katholieke Universiteit Leuven (KUL)
Status: Published in peer reviewed journal
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in vitro MDSC-T cell immunosuppression assay

Myeloid progenitor cells derived from the bone marrow of mice are stimulated using a cytokine mixture to become myeloid derived suppressor cell (MDSC) like cells. These are co-cultured with activated CD8+ T-cells derived from a mouse spleen. The MDSC like cells will suppress the proliferation of the

Last updated on: 10-01-2023 - 16:48

Contact: Yani Berckmans
Organisation: Katholieke Universiteit Leuven (KUL), Oncology - KU Leuven
Status: History of use, Published in peer reviewed journal
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Generation of Human Motor Units with Functional Neuromuscular Junctions in Microfluidic Devices

This study aimed to create a versatile and reproducible in vitro model of a human motor unit with functional neuromuscular junctions (NMJs). Therefore, human induced pluripotent stem cell (hiPSC)-derived motor neurons and human primary mesoangioblast (MAB)-derived myotubes were co-cultured in

Last updated on: 09-01-2023 - 15:13

Contact: Katarina Stoklund Dittlau
Organisation: Vlaams Instituut voor Biotechnologie (VIB), Katholieke Universiteit Leuven (KUL), Neurosciences - KU Leuven
Status: Published in peer reviewed journal
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Cold Atmospheric Plasma on mouse intestinal epithelial organoids

Using the ex vivo culture system, we investigated the impact of an endoscopic helium plasma jet application on mouse ISCs at the morphological, cellular and transcriptomic levels. Moreover, we explored the potential selectivity of CAP application on tumor versus normal organoids originating from the

Last updated on: 09-01-2023 - 09:42

Contact: Marie-Isabelle Garcia
Organisation: Université Libre de Bruxelles (ULB)
Status: Published in peer reviewed journal
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Development of luminescent human iPSC-derived neurospheroids

This method relates to the development of highly reproducible human iPSC-derived neurospheroids equipped with intrinsic bioluminescence for an easy and longitudinal follow-up of the viability and growth of these neurospheroids over time. The luminescent neurospheroids have been applied in ischemic

Last updated on: 02-01-2023 - 10:27

Contact: Elise Van Breedam
Organisation: University of Antwerp (UAntwerpen)
Status: Published in peer reviewed journal
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Glycogen storage assay

Cultivated liver cells are fixed with 4% (w/v) paraformaldehyde (PFA) for 10 minutes at room temperature and subsequently incubated for 15 minutes with 100 mM glycin solution, used to saturate reactive groups generated after PFA fixation. These cells are subsequently incubated for 10 minutes with 1%

Last updated on: 16-12-2022 - 19:40

Contact: Joery De Kock
Organisation: Vrije Universiteit Brussel (VUB)
Status: History of use, Internally validated, Published in peer reviewed journal
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Chicken embryonic spinal cord electroporation

The goal of chicken embryonic spinal cord electroporation is to increase or to reduce expression levels of genes of interest in the developing spinal cord, and to assess the phenotypic consequences of these alterations on neuronal differentiation or migration. Fertilized eggs stored at 14°C are

Last updated on: 13-12-2022 - 11:23

Contact: Frédéric Clotman
Organisation: Université Catholique de Louvain (UCL)
Status: History of use, Internally validated, Published in peer reviewed journal
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VERMEER FCM: a tool combining migration modelling and in silico hazard predictions for substances migrating from food contact materials

For the risk assessment of compounds migrating from food contact materials (FCM), information on the exposure to the migrant as well as its possible hazards is needed. To support the evaluation of both starting products and NIAS from plastic FCM, the VERMEER FCM tool has been developed within the

Last updated on: 29-11-2022 - 14:46

Contact: Ilse Van Overmeire
Organisation: Sciensano
Status: Internally validated
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