Functional cardiomyocytes can be efficiently derived from human pluripotent stem cells, which collectively include embryonic and induced pluripotent stem cells (iPSC). Specific affected biological pathways involved in disease can be functionally studied in differentiated cells at a single patient
Last updated on: 08-11-2019 - 11:01
FIBEr (Flanders Institute for Biomechanical Experimentation) is a laboratory that investigates the mechanical properties (stiffness, strength, microstructure) of biological tissue, in casu bones, muscles, ligaments, blood vessels, etc. A quantitative characterization of the mechanical behavior of
Last updated on: 12-11-2019 - 11:45
Contact: Nele Famaey
Organisation: Katholieke Universiteit Leuven (KUL)
Partners: Katholieke Universiteit Leuven (KUL)
Status: Still in development, History of use, Internally validated
Cell Culture and Reagents hiPSC-CMs can be obtained commercially either as living pre-plated cells seeded onto fibronectin-coated 96-well mClear plates (Greiner Bio-One, No. 655090) or can be plated in house at a density (~25,000 cells/well) suited to forming a confluent synchronously beating mono
Last updated on: 26-11-2019 - 13:17
Contact: Ard Teisman
Organisation: Janssen Pharma of JNJ
Status: Internally validated, Published in peer reviewed journal, Validated by an external party (e.g. OECD, EURL ECVAM,…)
The Koken rat is a dummy rat that is developed to train animal procedures, such as handling, blood sampling and intubation.
Last updated on: 15-10-2022 - 09:06
The DPRA is an in chemico method which quantifies the remaining concentration of cysteine- or lysine-containing peptide following 24 hours incubation with the test chemical at 25 +/-2,5ºC. The synthetic peptides contain phenylalanine to aid in the detection. Relative peptide concentration is
Last updated on: 22-02-2022 - 10:59
Contact: Bart Desmedt
Organisation: Sciensano
Status: History of use, Internally validated, Validated by an external party (e.g. OECD, EURL ECVAM,…)
The monocyte-activation test (MAT) is used to detect or quantify substances that activate human monocytes or monocytic cells to release endogenous mediators such as pro-inflammatory cytokines, for example tumour necrosis factor alpha (TNFα), interleukin-1 beta (IL-1β) and interleukin-6 (IL-6). These
Last updated on: 22-02-2022 - 10:56
HepG2 is a human hepatoma derived cell line, which are epithelial in morphology. It was established from liver tissue of a 15-year-old Caucasian male with a well differentiated hepatocellular carcinoma. The HepG2 cell line is one of the most used human liver-based in vitro models. The cells secrete
Last updated on: 28-02-2022 - 11:02
Human umbilical cord-derived mesenchymal stromal cells (hUC-MSCs) express several key liver-specific transcription factors as well as hepatic progenitor markers. However, they still lack the hepatocyte nuclear factors 1-alpha (HNF1a) and 4-alpha (HNF4a), indispensable for their reprogramming towards
Last updated on: 28-02-2022 - 11:02
Albumin secretion in the supernatant of in vitro cultures can be measured by an enzyme-linked immunosorbent assay. The assay is based upon a quantitative sandwich enzyme immunoassay technique and measures albumin in the culture medium of primary hepatocytes and/or hepatocyte like cells. Standards
Last updated on: 28-02-2022 - 11:02
Liver functionality can be monitored by the urea synthesis. In culture medium the measurement of urea synthesis relies on a chromogenic reagent that specifically forms a colored complex with urea. The concentration of this complex between urea, o-phthalaldehyde and N-(1-naphthyl) ethylenediamine can
Last updated on: 28-02-2022 - 11:03
