By the use of monolayer cultures as an in vitro system, the effects of drugs on CYP3A activity can be evaluated. It relies on the use of a luminogenic CYP3A substrate, namely luciferin-6′-pentafluorobenzylether (luciferin- PFBE). Upon biotransformation by CYP3A, luciferin-PFBE is converted into
Last updated on: 23-07-2025 - 09:53
Liver functionality can be monitored by the urea synthesis. In culture medium the measurement of urea synthesis relies on a chromogenic reagent that specifically forms a colored complex with urea. The concentration of this complex between urea, o-phthalaldehyde and N-(1-naphthyl) ethylenediamine can
Last updated on: 23-07-2025 - 09:53
The present standard procedure describes a protocol for measuring the urea concentration in supernatant of human stem cell-derived hepatocyte-like cells. This procedure relies on a chromogenic reagent that forms a colored complex specifically with urea. The latter can be measured and is directly
Last updated on: 23-07-2025 - 09:52
Contact: Cannot be disclosed
Organisation: Vrije Universiteit Brussel (VUB)
Status: History of use, Internally validated
The effect of fluidics that mimic the blood flow in the liver sinusoids, is evaluated during the hepatic differentiation of human skin-derived precursors (hSKP). In a standard bi-dimensional (2D) cell culture system, hSKP are differentiated to hSKP-HPC for 24 days in static conditions. In a
Last updated on: 23-07-2025 - 09:51
Contact: Robim Rodrigues
Organisation: Vrije Universiteit Brussel (VUB)
Status: Still in development
Polycaprolactone (PCL) fiber materials are fabricated using an electrospinning method. Molecular weight of PCL is 45 000 Da (Sigma-Aldrich). The electrospinning process is performed using 18wt% PCL solution dissolved in chloroform:ethanol at a ratio of 9:1. Two high-voltage sources are used to
Last updated on: 23-07-2025 - 09:50
For the generation of the human skin-derived precursors (hSKP) spheroids, ultra-low attachment 96-well plates, cell-repellent (Greiner) are used. hSKP cultured on tissue culture plates are detached with trypple (5 minutes) and collected in a tube. After centrifugation, hSKP are counted at the Burker
Last updated on: 23-07-2025 - 09:50
Contact: Robim Rodrigues
Organisation: Vrije Universiteit Brussel (VUB)
Status: Still in development
Drug-induced phospholipidosis (DIPL) is a metabolic disorder characterized by an excessive intracellular accumulation of phospholipids caused by cationic drugs. Hepatic cells derived from human skin are evaluated as an in vitro model to investigate DIPL and its mechanisms. Human skin stem cells
Last updated on: 23-07-2025 - 09:49
Contact: Cannot be disclosed
Organisation: Vrije Universiteit Brussel (VUB)
Status: History of use
Studying spermatogenesis in situ has led to the understanding that the 3D reorganization of testicular cells into an interstitial and tubular compartment is of enormous importance for germ cell differentiation. We will rely on 3D bioprinting technology which gives control over cell deposition and
Last updated on: 08-07-2025 - 10:26
Contact: Yoni Baert
Organisation: Vrije Universiteit Brussel (VUB)
Status: Still in development, Published in peer reviewed journal
The GENOMARK tool in metabolically competent human HepaRG cells is based on the transcriptomic results obtained with 12 genotoxic (in vivo positive results) and 12 non-genotoxic (in vivo negative results) reference compounds. Genotoxic compounds were selected to cover different mechanisms of action
Last updated on: 08-07-2025 - 10:07
Contact: Birgit Mertens
Organisation: Sciensano, Vrije Universiteit Brussel (VUB)
Status: Internally validated, Published in peer reviewed journal
This method uses human pluripotent stem cells (hPSCs), including embryonic and induced pluripotent stem cells, to generate cortical brain organoids and model brain tumorigenesis through targeted genetic manipulation. The aim is to create a physiologically relevant, in vitro 3D system that mimics
Last updated on: 06-05-2025 - 15:34
Contact: Diana Al Delbany
Organisation: Vrije Universiteit Brussel (VUB)
Status: History of use, Internally validated, Published in peer reviewed journal
