Adult intestinal stem cells are grown into closed 3D structures with protruding buds and a luminal compartment. These 3D structures are produced from the stem cells present in the base of crypts isolated from rectal mucosa samples that can be collected by suction or forceps biopsy. The crypts are
Last updated on: 22-02-2024 - 11:15
Contact: Anabela Ramalho
Organisation: Katholieke Universiteit Leuven (KUL)
Status: Published in peer reviewed journal
Endotoxins are lipopolysaccharides anchored at the outer membrane of gram-negative bacteria. These pyrogenic compounds can be introduced in pharmaceutical products during their manufacture and could induce severe physiological reactions in humans. This is why endotoxins are dosed, in order to assure
Last updated on: 28-09-2023 - 11:59
Contact: Flore Laurent
Organisation: Sciensano
Status: Internally validated, Validated by an external party (e.g. OECD, EURL ECVAM,…)
Safety Assessment of Compounds after In Vitro Metabolic Conversion Using Zebrafish Eleuthero Embryos
Zebrafish-based platforms have recently emerged as a useful tool for toxicity testing as they combine the advantages of in vitro and in vivo methodologies. Nevertheless, the capacity to metabolically convert xenobiotics by zebrafish eleuthero embryos is supposedly low. To circumvent this concern, a
Last updated on: 01-09-2023 - 09:55
Contact: Marie Pardon
Organisation: Katholieke Universiteit Leuven (KUL)
Status: Published in peer reviewed journal
The spatiotemporal distribution of fluorescent compounds was examined during 48 h after immersion (10 µM) or microinjection (2 mg/kg) in the pericardial cavity (PC), intraperitoneally (IP) and yolk sac (IY) of 3 dpf zebrafsh eleuthero-embryos. By modelling the fluorescence of whole-body contours
Last updated on: 01-09-2023 - 09:43
Contact: Marie Pardon
Organisation: Katholieke Universiteit Leuven (KUL)
Status: Published in peer reviewed journal
Immersion treatment: zebrafish embryos at the two-cell stage were immersed in Danieau’s medium containing the compound. A compound concentration of 10 µM and DMSO concentration of 0.1% (v/v) in a volume of 5 mL was used per well (6-well-plates). At 72 h, the zebrafish medium containing the compound
Last updated on: 01-09-2023 - 09:32
Contact: Marie Pardon
Organisation: Katholieke Universiteit Leuven (KUL)
Status: Published in peer reviewed journal
The method is an approach to systematically study the impact of the nutrient microenvironment on the metabolism of effector CD8+ T cells, based on performing stable 13C isotope labeling measurements on in vitro-differentiated murine effector CD8+ T cells. Naive CD8+ T cells are isolated from mouse
Last updated on: 31-08-2023 - 17:12
Contact: Dorien Broekaert
Organisation: Katholieke Universiteit Leuven (KUL), VIB - KU Leuven
Status: Published in peer reviewed journal
Metabolomics and 13C tracer analysis are state-of-the-art techniques that allow determining the concentration of metabolites and the activity of metabolic pathways, respectively. Three dimensional (3D) cultures of cancer cells constitute an enriched in vitro environment that can be used to assay
Last updated on: 31-08-2023 - 17:12
Contact: Dorien Broekaert
Organisation: VIB - KU Leuven, Katholieke Universiteit Leuven (KUL)
Status: Published in peer reviewed journal
Generally, our research group aims to study the influence of food parameters (food design, food processing, food composition) on the digestive kinetics of diverse nutrients throughout the upper gastrointestinal tract. For this, we use in vitro digestion protocols. The current method is a semi
Last updated on: 15-02-2023 - 16:08
Contact: Tara Grauwet
Organisation: Katholieke Universiteit Leuven (KUL)
Status: Published in peer reviewed journal
The purpose of the present development is to use avian MoDCs to implement a cellular platform to increase understanding of the immune responses induced by various antigens of interest (e.g. vaccine candidates) and evaluate their immunogenic potential. Considering the difficulty to work on dendritic
Last updated on: 03-02-2023 - 08:51
Ex-vivo tissue explants (precision cut tissue slices) prepared with the Krumdieck Tissue Slicer are living, three-dimensional tissue slices closely resemble the organ from which it is prepared, with all the cell types present in their original tissue-matrix configuration where physiological and
Last updated on: 31-01-2023 - 15:52
