Drug-induced phospholipidosis (DIPL) is a metabolic disorder characterized by an excessive intracellular accumulation of phospholipids caused by cationic drugs. Hepatic cells derived from human skin are evaluated as an in vitro model to investigate DIPL and its mechanisms. Human skin stem cells
Last updated on: 23-07-2025 - 09:49
Contact: Cannot be disclosed
Organisation: Vrije Universiteit Brussel (VUB)
Status: History of use
Studying spermatogenesis in situ has led to the understanding that the 3D reorganization of testicular cells into an interstitial and tubular compartment is of enormous importance for germ cell differentiation. We will rely on 3D bioprinting technology which gives control over cell deposition and
Last updated on: 08-07-2025 - 10:26
Contact: Yoni Baert
Organisation: Vrije Universiteit Brussel (VUB)
Status: Still in development, Published in peer reviewed journal
The GENOMARK tool in metabolically competent human HepaRG cells is based on the transcriptomic results obtained with 12 genotoxic (in vivo positive results) and 12 non-genotoxic (in vivo negative results) reference compounds. Genotoxic compounds were selected to cover different mechanisms of action
Last updated on: 08-07-2025 - 10:07
Contact: Birgit Mertens
Organisation: Sciensano, Vrije Universiteit Brussel (VUB)
Status: Internally validated, Published in peer reviewed journal
This method uses human pluripotent stem cells (hPSCs), including embryonic and induced pluripotent stem cells, to generate cortical brain organoids and model brain tumorigenesis through targeted genetic manipulation. The aim is to create a physiologically relevant, in vitro 3D system that mimics
Last updated on: 06-05-2025 - 15:34
Contact: Diana Al Delbany
Organisation: Vrije Universiteit Brussel (VUB)
Status: History of use, Internally validated, Published in peer reviewed journal
Cholestatic drug-induced liver injury (cDILI) is a frequent reason for drug failure and withdrawal during premarketing and postmarketing stages of drug development. Strategies for reliable detection of cDILI in early drug development are therefore urgently needed. The drug-induced cholestasis index
Last updated on: 23-04-2025 - 11:36
Contact: Cannot be disclosed
Organisation: Vrije Universiteit Brussel (VUB)
Status: Published in peer reviewed journal
Human embryonic kidney (HEK) 293 FT cells is a celline that is very easy to culture and is used to obtain high viral titers. “293” is a reference to the 293th experiment wherein the cell line was discovered. A transfection with an adenovirus type 5 DNA fragment took place, causing the cell line to
Last updated on: 28-01-2025 - 15:12
Total Parenteral Nutrition (TPN) can cause adverse effects, including metabolic disorders and liver injury. TPN-associated liver injury, known as intestinal failure-associated liver disease (IFALD), represents a significant problem affecting up to 90% of individuals receiving TPN. Despite numerous
Last updated on: 10-09-2024 - 13:54
Contact: Cannot be disclosed
Organisation: Vrije Universiteit Brussel (VUB)
Status: Published in peer reviewed journal
Adverse outcome pathway (AOP) networks are versatile tools in toxicology and risk assessment that capture and visualize mechanisms driving toxicity originating from various data sources. They share a common structure consisting of a set of molecular initiating events and key events, connected by key
Last updated on: 20-02-2024 - 09:27
Contact: Cannot be disclosed
Organisation: Vrije Universiteit Brussel (VUB)
Status: Published in peer reviewed journal
Drug-induced intrahepatic cholestasis (DIC) is a main type of hepatic toxicity that is challenging to predict in early drug development stages. Preclinical animal studies often fail to detect DIC in humans. In vitro toxicogenomics assays using human liver cells have become a practical approach to
Last updated on: 15-02-2024 - 11:59
Contact: Cannot be disclosed
Organisation: Vrije Universiteit Brussel (VUB)
Status: Internally validated, Published in peer reviewed journal
Alkaptonuria (AKU) is a rare inborn error of metabolism caused by a defective homogentisate 1,2-dioxygenase (HGD), an enzyme involved in the tyrosine degradation pathway. Loss of HGD function leads to the accumulation of homogentisic acid (HGA) in connective body tissues in a process called
Last updated on: 06-12-2023 - 14:45
Contact: Sien Lequeue
Organisation: Vrije Universiteit Brussel (VUB)
Partners: RWTH Aachen
Status: Published in peer reviewed journal
