By the use of monolayer cultures as an in vitro system, the effects of drugs on CYP3A activity can be evaluated. It relies on the use of a luminogenic CYP3A substrate, namely luciferin-6′-pentafluorobenzylether (luciferin- PFBE). Upon biotransformation by CYP3A, luciferin-PFBE is converted into
Last updated on: 23-07-2025 - 09:53
Liver functionality can be monitored by the urea synthesis. In culture medium the measurement of urea synthesis relies on a chromogenic reagent that specifically forms a colored complex with urea. The concentration of this complex between urea, o-phthalaldehyde and N-(1-naphthyl) ethylenediamine can
Last updated on: 23-07-2025 - 09:53
The present standard procedure describes a protocol for measuring the urea concentration in supernatant of human stem cell-derived hepatocyte-like cells. This procedure relies on a chromogenic reagent that forms a colored complex specifically with urea. The latter can be measured and is directly
Last updated on: 23-07-2025 - 09:52
Contact: Cannot be disclosed
Organisation: Vrije Universiteit Brussel (VUB)
Status: History of use, Internally validated
The effect of fluidics that mimic the blood flow in the liver sinusoids, is evaluated during the hepatic differentiation of human skin-derived precursors (hSKP). In a standard bi-dimensional (2D) cell culture system, hSKP are differentiated to hSKP-HPC for 24 days in static conditions. In a
Last updated on: 23-07-2025 - 09:51
Contact: Robim Rodrigues
Organisation: Vrije Universiteit Brussel (VUB)
Status: Still in development
Polycaprolactone (PCL) fiber materials are fabricated using an electrospinning method. Molecular weight of PCL is 45 000 Da (Sigma-Aldrich). The electrospinning process is performed using 18wt% PCL solution dissolved in chloroform:ethanol at a ratio of 9:1. Two high-voltage sources are used to
Last updated on: 23-07-2025 - 09:50
For the generation of the human skin-derived precursors (hSKP) spheroids, ultra-low attachment 96-well plates, cell-repellent (Greiner) are used. hSKP cultured on tissue culture plates are detached with trypple (5 minutes) and collected in a tube. After centrifugation, hSKP are counted at the Burker
Last updated on: 23-07-2025 - 09:50
Contact: Robim Rodrigues
Organisation: Vrije Universiteit Brussel (VUB)
Status: Still in development
The large (1mm diameter), easily accessible eggs of the frog Xenopus laevis (100-1000 eggs at once per frog) offer the opportunity to reconstitute cell cycle events in-vitro by generating cell-free extracts which retain all the biochemical components that regulate cell cycle progression, as well as
Last updated on: 22-07-2025 - 09:40
Contact: Martina Boiardi
Organisation: Katholieke Universiteit Leuven (KUL), Cellular and Molecular Medicine - KU Leuven
Status: History of use, Published in peer reviewed journal
We developed a biventricular statistical shape model (SSM) from high-resolution cardiac CT scans from 271 healthy individuals. Leveraging the diversity captured by our biventricular SSM, we created a synthetic cohort of anatomically detailed, high-resolution, biventricular meshes. The geometries in
Last updated on: 16-07-2025 - 10:01
Contact: Lore Van Santvliet
Organisation: Katholieke Universiteit Leuven (KUL)
Status: Published in peer reviewed journal
Generation of cortical organoids from patient and control hIPSC.
Last updated on: 16-07-2025 - 10:01
Contact: Sarah Weckhuysen
Organisation: Vlaams Instituut voor Biotechnologie (VIB), University of Antwerp (UAntwerpen)
Status: Internally validated
We generate iPSC derived neurons and astrocytes using NgN2, ASCL1 and SOX9 overexpression respectively.
Last updated on: 16-07-2025 - 10:00
Contact: Sarah Weckhuysen
Organisation: Vlaams Instituut voor Biotechnologie (VIB), University of Antwerp (UAntwerpen)
Status: Published in peer reviewed journal
