In vitro gastrointestinal Dialysis model (with colon phase)
Commonly used acronym: GIDM & GIDM-colon
Scope of the method
- Animal health
- Human health
- Basic Research
- In vitro - Ex vivo
- Other (e.g. bacteria)
Description
- passive diffusion
- availability
- validated in vitro model
- colonic metabolism
- effect of food matrix on availability of compounds
- metabolisation pattern of bio-active compounds
- availability and release of compounds in pharmaceutical formula
The GIDM-colon is a validated in vitro model that allows the study of the availability of compounds and the metabolisation at the level of the colon. The human physiological conditions of the gastrointestinal tract (stomach, small intestine and colon) are mimicked. The continiuous flow eliminates the compounds diffusing through a semi-permeable membrane, simulating absorption by passive diffusion. The impact of the digestive conditions of various age groups or diseases states (e.g. metabolic syndrome) on availability and metabolite formation can be investigated.
- Anaerobic glove box ;
- Sample analysis:
- - HPLC,
- - AAS,
- - LC/QTOF.
- History of use
- Internally validated
- Published in peer reviewed journal
Pros, cons & Future potential
- Reproducability ;
- Circumstances can be well controlled and standardized ;
- Representative of colon microbiome ;
- Sampling at any time.
Absence of an active transport of digestion products.
An adaptation can be made to the gastrointestinal tract of animals investigating the availability and the release of compounds in certain drugs or healthy food.
References, associated documents and other information
Optimization of an in vitro gut microbiome biotransformation platform with chlorogenic acid as model compound: From fecal sample to biotransformation product identification. Mortelé O, Iturrospe E, Breynaert A, Verdickt E, Xavier BB, Lammens C, Malhotra-Kumar S , Jorens PG, Pieters L, van Nuijs ALN, Hermans N ; J Pharm Biomed Anal.2019 ; 175.
Chlorogenic Acid as a Model Compound for Optimization of an In Vitro Gut Microbiome-Metabolism Model. Mortelé O, Iturrospe E, Breynaert A, Xavier BB, Lammens C, Malhotra-Kumar S , Jorens PG, Pieters L, van Nuijs ALN, Hermans N: proceedings 2019, 11(1), 31.
Revelation of the metabolic pathway of hederacoside C using an innovative data analysis strategy for dynamic multiclass biotransformation experiments Peeters L, Beirnaert C, Van der Auwera A, Bijttebier S, de Bruyne T, Laukens K, Pieters L, Hermans N, Foubert K. Journal of chromatography: 2019, p. 240-247.
Toll-like receptor-dependent immunomodulatory activity of Pycnogenol® Verlaet A, Bolt van der N, Meijer B, Breynaert A, Naessens T, Konstanti P, Smidt H, Hermans N, Savelkoul H F.J., Teodorowicz M, Nutrients - ISSN 2072-6643-11:2. 2019.
In vitro gastrointestinal biotransformation of a Desmodium adscendens decoction: the first step in unravelling its behavior in the human body. van Dooren I, Foubert K, Bijttebier S, Breynaert A, Theunis M, Exarchou V, Claeys M, Hermans N, Apers S, Pieters L, Journal of Pharmacy and Pharmacology ISSN 0022-3573-70:10 p. 1414-1422. 2018.
In vitro and in vivo study of the gastrointestinal absorption and metabolisation of hymenocardine, a cyclopeptide alkaloid. Tuenter E, Bijttebier S, Foubert K, Breynaert A, Apers S, Hermans N, Pieters L; Planta medica: natural products and medicinal plant research, 83:9, p. 790-796. 2017.
Vegetable relishes, high in β-carotene, increase the iron, zinc and β-carotene nutritive values from cereal porridges. Kruger J, Breynaert A, Pieters L, Hermans N; International journal of food sciences and nutrition - ISSN 0963-7486, p. 1. 2017.
Development and validation of an in vitro experimental GastroIntestinal Dialysis Model with colon phase to study the availability and colonic metabolisation of polyphenolic compounds. Breynaert A, Bosscher D, Khant A, Claeys M, Cos P, Pieters L., Hermans N; Planta Medica, 81, 1075-1083), 2015.
Contact person
Annelies BreynaertOrganisations
University of Antwerp (UAntwerpen)Pharmaceutical Sciences
NatuRA (Natural Products and Food - Research and Analysis)
Belgium