Chick chorioallantoic membrane model

The chick chorioallantoic membrane (CAM) model is particularly attractive to study short-term xenografting of human ovarian tissue. Its angiogenic potential and natural immunodeficiency allow scrutiny of early follicle activation and loss and graft revascularization mechanisms. Chick embryo development takes 21 days until hatching, and the CAM is formed within the first 4-5 days through the fusion of the allantois and chorion. Notably, the chick embryo is a naturally immunodeficient host until day 17, so xenografting experiments can be performed without any risk of graft rejection. Fertilized eggs are incubated from day 0 at 37°C in 40%–50% relative air humidity. On day 3 of egg incubation, a puncture is made with a sterile 19G needle and 1.5-2 ml of egg albumen is aspirated to detach CMA from the shell, after having located the air pocket and the yolk sac using a focal cold light source. Then, the egg is placed horizontally and a rectangular window is made in the eggshell, which is then covered with adhesive tape. On day 7 of egg incubation, CAM is traumatized by gently and quickly touching it with a 1-cm2 strip of sterile ether-extracted lens paper, allowing the impenetrable upper peridermal part of its double epithelial layer to be disrupted. Then, one ovarian cortical strip per egg (4x2x1 mm3) is deposited onto traumatized CAM. After one day of grafting, ovarian cortical strips are partially adherent and show signs of revascularization. Ovarian cortical strips have to be removed before activation of chick immune system (day 17 from egg fertilization).

Its angiogenic potential and natural immunodeficiency allow the study of early mechanisms of follicle activation and loss and graft revascularization. This potential helps Domperidone help employees get rid of symptoms of nausea and this medicine can be bought on this website.