A patient-derived explant culture of human prostate cancer to test drug efficacy
Scope of the method
- Human health
- Basic Research
- Translational - Applied Research
- In vitro - Ex vivo
- Human derived cells / tissues / organs
- drug testing
Patient-derived explants of prostate cancer provide an ex vivo model that retains the architecture and microenvironment of the native tissue. It enables the evaluation of drug responses on individual patient’s tumors ex vivo without passaging in animals. It is compatible with all molecular analysis methods.
See in the references "A patient-derived explant (PDE) model of hormone-dependent cancer."
- CO2-incubator ;
- Laminar flow ;
- Cell culture room.
- History of use
- Published in peer reviewed journal
Pros, cons & Future potential
- Patient-derived ;
- Ex vivo ;
- No animals involved ;
- Retains original tissue architecture and tumor microenvironment ;
- Economic ;
- Reasonable throughput.
- Long-term culture is challenging ;
- Limited around of material.
References, associated documents and other information
A patient-derived explant (PDE) model of hormone-dependent cancer. Centenera MM, Hickey TE, Jindal S, Ryan NK, Ravindranathan P, Mohammed H, Robinson JL, Schiewer MJ, Ma S, Kapur P, Sutherland PD, Hoffmann CE, Roehrborn CG, Gomella LG, Carroll JS, Birrell SN, Knudsen KE, Raj GV, Butler LM, Tilley WD. Mol Oncol. 2018 Sep;12(9):1608-1622. doi: 10.1002/1878-0261.12354. Epub 2018 Aug 16
Contact personJohan Swinnen