Reconstruction of Human Epidermis in Culture

Commonly used acronym: RHE

Scope of the method

The Method relates to
  • Human health
The Method is situated in
  • Basic Research
Type of method
  • In vitro - Ex vivo
This method makes use of
  • Human derived cells / tissues / organs
Specify the type of cells/tissues/organs
Human epidermal keratinocytes


Method keywords
  • skin
  • epidermis
  • Reconstructed human epidermis
  • cutaneous toxicology
  • epidermal irritation
  • epidermal infection
Scientific area keywords
  • skin biology
  • dermatology
  • Infection models
Method description
  • Method to culture human epidermal keratinocytes and seed them for tissue reconstruction at the air-liquid interface over a polycarbonate porous membrane.
Lab equipment
  • - Culture hood,
  • - Culture incubator,
  • - Refrigerated centrifuge,
  • - Volt-ohm meter,
  • - Inverted phase-contrast microscope.
Method status
  • Published in peer reviewed journal

Pros, cons & Future potential

  • - This method allows production of human epidermal organoids,
  • - Other cell types like melanocytes can be added to the reconstruction,
  • - It allows studies of epidermal barrier in normal and pathological conditions.
  • There is no immune cell of the adaptative system in the model.
  • This reconstruction can be performed over synthetic dermis.
Future & Other applications
  • The model is increasingly used to mimick epidermal pathologies, either inflammatory, infectious, or cancerous.

References, associated documents and other information

Associated documents
De Vuyst 2014 Epidermal cells 191.pdf

Contact person

Yves Poumay


Université de Namur (UNamur)