The Evaluation of DNA-adduct Formation through DNA-Adductomics

Commonly used acronym: DNA adductomics

Scope of the method

The Method relates to
  • Animal health
  • Environment
  • Human health
The Method is situated in
  • Basic Research
  • Translational - Applied Research
Type of method
  • In chemico
DNA-Adductomics
This method makes use of
  • Animal derived cells / tissues / organs

Description

Method keywords
  • DNA damage
  • DNA adductomics
  • mass spectrometry
  • Liquid chromatography
  • metabolomics
Scientific area keywords
  • analytical chemistry
  • cancer research
  • genotoxicity and carcinogenicity
  • red meat consumption
  • food safety
  • mycotoxins
Method description

It is the goal of the DNA-adductomics to search for DNA-adducts that might be formed during interaction with contaminants. The analysis of DNA adducts is performed using ultra-high performance liquid chromatography coupled to hybrid quadrupole-Orbitrap high resolution mass spectrometry. Both the instrumental method, as well as generic extraction protocol have been extensively validated and enable both a targeted as well as an untargeted DNA adduct analysis. The metabolomics workflow consists of a sample preparation, followed by the UPHLC-HRMS analysis, after which multivariate statistical analysis will be performed to identify DNA-adducts.

Lab equipment
  • UHPLC ;
  • HR-Otrbitrap-MS.
Method status
  • Internally validated
  • Published in peer reviewed journal

Pros, cons & Future potential

Advantages
  • Investigation of DNA adduct formation can provide valuable information on exposure to both environmental and endogenous chemicals with genotoxic, mutagenic and/or carcinogenic properties on the one hand, and their possible adverse health effects on the other.
  • DNA adduct analysis can be very useful to investigate the underlying pathways of several non-hereditary cancers, which comprise the vast majority of cancer cases.
Challenges
  • Multi-step procedure => Long analysis time, extensive sample preparation ;
  • Big data handling.

References, associated documents and other information

References

Vanden Bussche et al (2012) Journal of Chromatography A, 1257, 25-33 L.Y.

Hemeryck et al (2015) Analytica Chimica Acta, 892, 123-131 L.Y.

Hemeryck et al (2016) Analytical Chemistry, 88, 7436-7446 L.Y.

Hemeryck et al (2017) Food Chemistry, 230, 378-387 L.Y.

Hemeryck et al (2018) Food and Chemical Toxicology, 115, 73-87

Associated documents
Vanden Bussche et al, 2012.pdf
Hemeryck et al, 2017.pdf
Hemeryck et al, 2018.pdf
Hemeryck et al, 2015.pdf
Hemercyk et al, 2016.pdf
Links
Vanden Bussche et al, 2012
L.Y. Hemeryck et al, 2015
L.Y. Hemeryck et al, 2016
L.Y. Hemeryck et al, 2017
L.Y. Hemeryck et al, 2018

Contact person

Lynn Vanhaecke

Organisations

Ghent University
Faculty of Veterinary Medicine, Department of Veterinary Public Health and Food Safety
Belgium
Flemish Region