The HepaRG cell line: a unique in vitro hepatic cell system

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Scope of the method

The Method relates to

Human health

The Method is situated in

Basic Research

Type of method

In vitro - Ex vivo

This method makes use of

Human derived cells / tissues / organs

Specify the type of cells/tissues/organs

Terminally differentiated human hepatocellular carcinoma cells

Description

Method keywords

cell culture
in vitro tool
broad application
hepatic cell line
ready-to-use

Scientific area keywords

Liver cell biology
Toxicity studies
Drug metabolism
genotoxicity and carcinogenicity
hepatotoxicity screening hepatotoxicity screening

Method description

Cryopreserved differentiated HepaRG cells (obtained from Biopredic International) are derived from a human hepatocellular carcinoma. These cells are an unique in vitro tool that provides reproducible results and exhibit many characteristics of primary human hepatocytes such as morphology, expression of key metabolic enzymes, nuclear receptors and drug transporters. Because of these characteristics they have a very broad application versatility like in vitro ADME, hepatotoxicity screening and mechanistic testing applications (for instance transporters, drug-induced liver injury, genotoxicity and carcinogenicity studies).

Lab equipment