Immune phenotyping in the peripheral blood
Scope of the method
- Human health
- Basic Research
- In vitro - Ex vivo
- Human derived cells / tissues / organs
- flow cytometry
- peripheral blood mononuclear cells
- immune cell phenotyping
- human white blood cells
Peripheral blood mononuclear cells (PBMC) are isolated from the peripheral blood using Ficoll gradient centrifugation. The PBMC are then stained with fluorescently-labelled monoclonal antibodies directed against cell surface or intracellular proteins that can be used to identify specific immune cell subtypes. Hereby, T cell subpopulations, B cell subpopulations, NK cell subpopulations and monocytes can be discriminated.
- Published in peer reviewed journal
Pros, cons & Future potential
Additional surface or intracellular markers can be added.
References, associated documents and other information
Biomedical Research Institute