MISpheroID: a knowledgebase and transparency tool for minimum information in spheroid identity
Commonly used acronym: MISpheroID
Scope of the method
- Basic Research
- Education and training
- Translational - Applied Research
- Animal derived cells / tissues / organs
- 3D cel culture
- in vitro 3D modelling
Spheroids are three-dimensional cellular models with widespread basic and translational application across academia and industry. However, methodological transparency and guidelines for spheroid research have not yet been established. The MISpheroID Consortium developed a crowdsourcing knowledgebase that assembles the experimental parameters of 3,058 published spheroid-related experiments. Interrogation of this knowledgebase identified heterogeneity in the methodological setup of spheroids. Empirical evaluation and interlaboratory validation of selected variations in spheroid methodology revealed diverse impacts on spheroid metrics. To facilitate interpretation, stimulate transparency and increase awareness, the Consortium defines the MISpheroID string, a minimum set of experimental parameters required to report spheroid research. Thus, MISpheroID combines a valuable resource and a tool for three-dimensional cellular models to mine experimental parameters and to improve reproducibility.
- Published in peer reviewed journal
Pros, cons & Future potential
Thus, MISpheroID is a unique open-access resource that facilitates systematic reporting on essential spheroid methodology with the aim to increase consistency and awareness in both academic and industrial research environments.
Keep it updated and keep it active in the long term.
Include additional essential spheroid methodological aspects based on empirical evidence.
Other emerging 3D model systems, such as organoids and self-renewing multicellular aggregates that self-organize into lumen-containing ex-vivo organs, may also benefit from the MISpheroID tool to improve transparency and document heterogeneity.
References, associated documents and other information
Peirsman, A.; Blondeel, E.; MISpheroID consortium; De Wever, O. (2021) MISpheroID: a knowledgebase and transparency tool for minimum information in spheroid identity. Nat. Methods 18, 1294–1303
Contact personOlivier De Wever
OrganisationsGhent University (UGent)
Department of Human Structure and Repair