MISpheroID: a knowledgebase and transparency tool for minimum information in spheroid identity

Commonly used acronym: MISpheroID

Scope of the method

The Method relates to
  • Other
transparency and reproducibility in 3D culture conditions such as spheroids, organoids, etc
The Method is situated in
  • Basic Research
  • Education and training
  • Translational - Applied Research
Type of method
  • Other


Method keywords
  • 3D cel culture
  • spheroids
  • organoids
  • Reproducibility
  • transparency
  • standardization
Scientific area keywords
  • spheroids
  • reproducibility
  • in vitro 3D modelling
  • transparency
  • standardization
Method description

Spheroids are three-dimensional cellular models with widespread basic and translational application across academia and industry. However, methodological transparency and guidelines for spheroid research have not yet been established. The MISpheroID Consortium developed a crowdsourcing knowledgebase that assembles the experimental parameters of 3,058 published spheroid-related experiments. Interrogation of this knowledgebase identified heterogeneity in the methodological setup of spheroids. Empirical evaluation and interlaboratory validation of selected variations in spheroid methodology revealed diverse impacts on spheroid metrics. To facilitate interpretation, stimulate transparency and increase awareness, the Consortium defines the MISpheroID string, a minimum set of experimental parameters required to report spheroid research. Thus, MISpheroID combines a valuable resource and a tool for three-dimensional cellular models to mine experimental parameters and to improve reproducibility.

Lab equipment


Method status
  • Published in peer reviewed journal

Pros, cons & Future potential


Thus, MISpheroID is a unique open-access resource that facilitates systematic reporting on essential spheroid methodology with the aim to increase consistency and awareness in both academic and industrial research environments.


Keep it updated and keep it active in the long term.


Include additional essential spheroid methodological aspects based on empirical evidence.

Future & Other applications

Other emerging 3D model systems, such as organoids and self-renewing multicellular aggregates that self-organize into lumen-containing ex-vivo organs, may also benefit from the MISpheroID tool to improve transparency and document heterogeneity.

References, associated documents and other information


Peirsman, A.; Blondeel, E.; MISpheroID consortium; De Wever, O. (2021) MISpheroID: a knowledgebase and transparency tool for minimum information in spheroid identity. Nat. Methods 18, 1294–1303

Associated documents
MISpheroID: a knowledgebase and transparency tool for minimum information in sp…

Contact person

Olivier De Wever


Ghent University (UGent)
Department of Human Structure and Repair
Flemish Region