A model of inflamed primary human synoviocytes for the evaluation of compounds in the physiopathology of joint diseases
Commonly used acronym: Model of inflamed synoviocytes
Scope of the method
- Human health
- Translational - Applied Research
- In vitro - Ex vivo
- Human derived cells / tissues / organs
Description
- inflammation
- cartilage catabolism
- short-term
- mode of action
- screening
- joint health
- anti-inflammatory properties
- medical devices
- visco-supplements
- anti-catabolic properties
- osteoarthritis
- drugs
- food supplements
The culture of primary human synoviocytes provides an excellent cellular model for studying the normal and pathological physiology of synoviocytes and the development of joint diseases. Human primary synoviocytes can either be provided by commercial suppliers or isolated from fresh biological material (synovial membrane tissue sampled during total knee replacement surgeries). Primary synoviocytes are cultured in monolayer, for short-term periods (usually from 24h to 72h), in presence of IL-1b which is efficient to induce a pro-inflammatory environment and pro-catabolic conditions. This short-term model is used to assess the mode of action of several novel therapeutic solutions intended for joint diseases such as osteoarthritis (drugs, food supplements, medical devices, ATMP). It can also be used as a rapid screening model before moving on to in vivo experiments (in view of the 3Rs). Dexamethasone is used as positive control to counter the pro-inflammatory and pro-catabolic status of the inflamed synoviocytes.
- - Laminar flow hood and CO2 incubator (for cell culture),
- - qPCR machine (for molecular biology analyses),
- - Spectrophotometer and fluorometer (for NO and DNA measurements, respectively).
- History of use
- Internally validated
Pros, cons & Future potential
- - Short-term, cheap and efficient screening model,
- - Easy access to the HFLS (commercial supplier).
- Inter-variability between donors (primary cultures).
- This model could be adapted into a co-culture model with chondrocytes to better mimic the joint articulation.
- This model is currently adapted to other species in our facilities (dog, horse, ...) for the testing of veterinary products.
References, associated documents and other information
Contact person
Julie UerlingsOrganisations
ARTIALISPreclinical Department
Belgium
Walloon