The Spheroid Light Microscopy Image Atlas for morphometrical analysis of three-dimensional cell cultures

Commonly used acronym: SLiMIA

Scope of the method

The Method relates to
  • Other
Morphological analysis
The Method is situated in
  • Translational - Applied Research
Type of method
  • In silico

Description

Method keywords
  • Image analysis
  • spheroids
  • morphological analysis
  • image atlas
  • 3D cel culture
Scientific area keywords
  • 3D in vitro models
  • cancer
  • Translational Research
  • biomedical research
  • Microscopy
Method description
Here, we introduce an open-access Spheroid Light Microscopy Image Atlas (SLiMIA) that can serve as a training set for morphology studies of 3D cell cultures. We provide images with a variety of metadata: 9 microscopes, 47 cell lines, 8 culture media, 4 spheroid formation methods and multiple cell seeding densities; totalling approximately 8,000 images of spheroids. This comprehensive dataset can guide spheroid researchers and promote economizing of resources by advancing 3D cell culture optimization, standardization and implementation by the community at large. Considering the exponentially growing interest in spheroid morphometrical analyses and the emerging technological possibilities to do so, this atlas can be applied to train and develop image segmentation models to deepen our understanding of 3D spheroid morphometry in biomedical research.
Method status
  • Published in peer reviewed journal

Pros, cons & Future potential

Advantages

SLiMIA is a dataset comprising approximately 8,000 images and has an enormous potential to assist in the development, training and validation of spheroid segmentation models and to connect spheroid morphometry with methodological metadata.

Future & Other applications

SLiMIA can be applied to compare spheroid diversity and to develop morphometric analysis software with potential applications on other 3D cultures such as organoids and patient-derived tissue fragments.

References, associated documents and other information

Associated documents
SLiMIA publication.pdf
Links
The Spheroid Light Microscopy Image Atlas for morphometrical analysis of three-…

Contact person

Eva Blondeel

Organisations

Ghent University (UGent)
Department of Human Structure and Repair
Laboratory of Experimental Cancer Research
Belgium
Flemish Region