Cardiomyocytes derived from induced pluripotent stem cells (iPSC-CMs) offer an attractive platform for cardiovascular research, including disease modeling, drug toxicity testing and development of regenerative therapies. Patient-specific iPSC-CMs are very useful to study disease pathogenesis and
Last updated on: 30-06-2020 - 14:13
Contact: Maaike Alaerts
Organisation: University of Antwerp
Status: Still in development, Internally validated
Vascular smooth muscle cell (VSMC) deficiency plays a pivotal role in aneurysm development. Unfortunately, access to native VSMCs of patients and (particularly) control individuals is extremely limited. It has been shown that iPSC-derived VSMCs recapitulate the yet known disease processes very well.
Last updated on: 03-06-2020 - 13:51
Contact: Aline Verstraeten
Organisation: University of Antwerp
Status: Still in development, Internally validated
This method is a straightforward, colorimetric, and inexpensive high-throughput screening system in bacteria which depends on the activity of human HPD. This screening assay is based on the formation and accumulation of a melanin-like ochronotic pigment which has a characteristic brown color. In the
Last updated on: 26-05-2020 - 11:30
Contact: Jessie Neuckermans
Organisation: Vrije Universiteit Brussel, RWTH Aachen
Partners: RWTH Aachen
Status: Published in peer reviewed journal
Amoebae are natural eukaryotic predators of bacteria, yeasts, fungi and they are ubiquiste. They are excellent and easy-to-use cellular infection models, as they allow to co-cultivate any organisms in a broad range of infection medium, compatible with high quality microscopy techniques,
Last updated on: 19-05-2020 - 11:02
Contact: Charles Van der Henst
Organisation: Vrije Universiteit Brussel
Status: Internally validated, Published in peer reviewed journal
A calculated number of bacteria is injected through the pro-legs of the larvae. As several entry points are present, this allows to inject virulent bacteria and potential antimicrobial compounds within the same host. The survival of the larvae is scored over time (days post inoculation) until about
Last updated on: 19-05-2020 - 11:01
Contact: Charles Van der Henst
Organisation: Vrije Universiteit Brussel
Status: Internally validated, Published in peer reviewed journal
Reverse pharmacology is a high-throughput in vitro method to characterise ligand-receptor interactions. In this method, a receptor of interest is expressed in a heterologous cell line and used as a hook to fish out its ligand(s) from a library of synthetic compounds. Receptor activation is measured
Last updated on: 17-04-2020 - 09:56
The nematode Caenorhabditis elegans provides a powerful model system to study fundamental working mechanisms of the nervous system in a living animal. It's main advantages are its compact nervous system that has been fully mapped, its short generation time and amenability for genetic research. As a
Last updated on: 17-04-2020 - 09:55
Performing biopredictive dissolution tests in in vitro models that are frequently used in pharmaceutical and academic institutions and using these in vitro dissolution data as input for PBPK models to predict the systemic exposure of the drug in humans/patients.
Last updated on: 08-04-2020 - 17:29
We study the influence of food parameters on the digestive kinetics of diverse nutrients throughout the upper gastrointestinal tract. For this, we use a static in vitro digestion protocol based on the standardized, consensus method recommended by the INFOGEST consortium. Briefly, the food is first
Last updated on: 31-03-2020 - 21:01
Contact: Tara Grauwet
Organisation: KU Leuven
Partners: INFOGEST consortium
Status: Published in peer reviewed journal
The GENOMARK tool in metabolically competent human HepaRG cells is based on the transcriptomic results obtained with 12 genotoxic (in vivo positive results) and 12 non-genotoxic (in vivo negative results) reference compounds. Genotoxic compounds were selected to cover different mechanisms of action
Last updated on: 27-03-2020 - 17:23
Contact: Jolien Van De Maele
Organisation: Sciensano
Partners: Vrije Universiteit Brussel
Status: Internally validated, Published in peer reviewed journal
