A battery of in vitro genotoxicity assays is currently used to detect agents with DNA damaging and carcinogenic potential. Although the sensitivity of this battery of genotoxicity assays is high, the specificity is low (“false positive hit”), especially with the mammalian cell-based assay, compared
Last updated on: 11-07-2022 - 15:51
Contact: Marc Audebert
Organisation: Institut national de la recherche agronomique (INRAE)
Status: History of use, Internally validated, Published in peer reviewed journal, Currently submitted for further validation by an external party (e.g. OECD, EURL ECVAM,…)
DARTpaths is an an integrative app to support the prioritisation of chemicals. The Open Source R shiny application allows for the prediction of compound-induced molecular mechanisms of action. The tool integrates phenotypic endpoints of different species induced by compounds and genetic variants, in
Last updated on: 13-06-2022 - 16:04
Contact: Vera van Noort
Organisation: Katholieke Universiteit Leuven (KUL)
Partners: Open Analytics, Hogeschool Utrecht , Vivaltes
Status: Internally validated
In vitro differentiation of hematopoietic stem cells (HSC) or inducible pluripotent stem cells (IPS) to megakaryocytes and platelets using specific differentiation conditions (liquid and 3D media). CRISPR/cas mutagenesis of HSC or IPS to study the effect of gene depletion or specific mutants on
Last updated on: 26-05-2022 - 10:41
Contact: Kathleen Freson
Organisation: Katholieke Universiteit Leuven (KUL)
Status: Still in development, Internally validated, Published in peer reviewed journal
The VITOTOX test is a bacterial genotoxicity test. The test is based on bacteria that contain the lux operon of Vibrio fischeri under transcriptional control of the mutated recN promoter, which is controlled by the bacterial SOS-system (TA 104-recN2-4 strain or Genox strain). After incubation
Last updated on: 26-05-2022 - 10:40
Contact: Roel Anthonissen
Organisation: Sciensano
Status: History of use, Published in peer reviewed journal
This method uses human skin-derived precursors (hSKP) differentiated towards hepatic cells (hSKP-HPC) as a hepatic in vitro model. Exposure of these cells for 24 hours to sub-cytotoxic concentrations of acetaminophen, which is a reference hepatotoxicant, induced specific cellular responses in a
Last updated on: 26-05-2022 - 10:39
Contact: Robim Rodrigues
Organisation: Vrije Universiteit Brussel (VUB)
Status: Published in peer reviewed journal
The CALUX® system (Chemically Actived LUciferase eXpression) of Bio Detection Systems (BDS, Amsterdam, the Netherlands) uses U-2 OS cells (human osteoblast) that are stably transfected with human TRb or human PPARg2 (BDS, Amsterdam) and a luciferase reporter construct under the control of a receptor
Last updated on: 05-05-2022 - 08:59
Contact: Cannot be disclosed
Organisation: Sciensano
Partners: BDS
Status: Published in peer reviewed journal
Recent advances in the development of human-based in vitro models offer new tools for drug screening and mechanistic investigations of new therapeutic agents. However, there is a lack of evidence that disease models respond favourably to potential drug candidates. Atopic dermatitis (AD) is a very
Last updated on: 22-04-2022 - 09:17
Contact: Christel Boudry
Organisation: straticell
Status: Internally validated, Published in peer reviewed journal
This method provides a rapid untargeted screening metabolomics method, which can be used to evaluate whether or not metabolic changes can be appointed according to the metabolic state in your matrix of choice. It applies an ambient ionization technique in which a laser beam or diathermy probe is
Last updated on: 31-03-2022 - 15:31
Contact: Lynn Vanhaecke
Organisation: Ghent University (UGent)
Status: Internally validated, Published in peer reviewed journal
OECD TG 249 was developed by Prof. Dr. Kristin Schirmer, from Eawag. The OECD 249 aims at assessing the acute toxicity of single or mixtures of chemicals using the permanent rainbow trout gill cell line, RTgill-W1. RTgill-W1 cells in a confluent monolayer in 24-well plates are exposed to the test
Last updated on: 30-03-2022 - 17:15
Contact: João Barbosa
Organisation: Ghent University (UGent)
Status: Published in peer reviewed journal, Validated by an external party (e.g. OECD, EURL ECVAM,…)
In silico tools are computer-assisted methodologies with a high-throughput that allow to predict the toxic potential of compounds without experimental testing. Consequently, in silico tools are time-, cost- and animal-saving in nature. The most commonly used methods are (quantitative) structure
Last updated on: 24-03-2022 - 11:25
