We propose to generate three-dimensional tumoroids from the primary melanoma cell lines, as well as 3D organoids from the iPSc-derived neural crest stem cells. We will use the AggreWell system (STEMCELL Technologies) to generate uniform, size-controlled three-dimensional spheroids. After 5 days in

Last updated on: 25-02-2020 - 23:58

Organisation: VIB KU Leuven
Status: Published in peer reviewed journal
Cell line which is commonly used to study biological processes in mouse macrophages. It is a macrophage-like cell line derived from BALB/c mice.

Last updated on: 25-02-2020 - 23:58

Contact: Jerome Hendriks
Organisation: Hasselt University
Status: History of use
Organotypical culture models, such as retinal explants, is an ideal alternative for in vitro retinal cell cultures and preclinical animal models, as they provide the necessary compromise between these two model systems. The major advantage of explant cultures is that cells are kept within their

Last updated on: 21-02-2020 - 14:51

Contact: Evy Lefevere
Organisation: KU Leuven
Status: Internally validated, Published in peer reviewed journal
The cardiorespiratory model reproduces human physiology with a high level of fidelity. It is a lumped parameter model including a representation of atria, ventricles, pulmonary and systemic circulations, autonomic controls, metabolic peripheral control, ventilation and gas exchange in tissues and

Last updated on: 14-02-2020 - 12:54

Contact: Libera Fresiello
Organisation: Cannot be disclosed
Partners: Cannot be disclosed
Status: History of use, Internally validated, Published in peer reviewed journal
We have developed and validated yeast models to study aspects related to protein folding diseases like Alzheimer's and Parkinson's disease. These models allow to gain further insight in the cellular processes involved in the etiology of these disorders and as such identify potential new bio-markers

Last updated on: 11-02-2020 - 11:17

Contact: Joris Winderickx
Organisation: KU Leuven
Status: Published in peer reviewed journal
The method enables to quantitatively assess the invasion of endothelial cells in extracellular-matrix mimicking hydrogels, such as collagen or polyethylene glycol, and to measure the forces exerted by the cells that enable them to invade. Endothelial cells are seeded on the side of a hydrogel and

Last updated on: 11-02-2020 - 08:55

Organisation: KU Leuven
Status: Published in peer reviewed journal
Patient-derived explants of prostate cancer provide an ex vivo model that retains the architecture and microenvironment of the native tissue. It enables the evaluation of drug responses on individual patient’s tumors ex vivo without passaging in animals. It is compatible with all molecular analysis

Last updated on: 07-02-2020 - 15:55

Contact: Johan Swinnen
Organisation: KU Leuven
Partners: University of Adelaide
Status: History of use, Published in peer reviewed journal
Ex vivo lung perfusion (EVLP) is a form of isolated lung perfusion in normothermic conditions and can be achieved with a pump-driven perfusion machine that recirculates a preservation solution through the vasculature of the lung in addition to mechanical ventilation. EVLP is based on a physiological

Last updated on: 07-02-2020 - 15:50

Contact: Arne Neyrinck
Organisation: KU Leuven
Status: Published in peer reviewed journal
The feed undergoes an in vitro enzymatic hydrolysis simulating the digestion in the upper digestive tract followed by a dialysis mimicking the intestinal absorption. Finally, an in vitro batch fermentation is performed and simulates the fermentation occurring in the hindgut. The ingredients, ground

Last updated on: 04-02-2020 - 13:36

Contact: Nadia Everaert
Organisation: Liège Université
Status: Published in peer reviewed journal
We have developed an innovative two-chamber skin explant model specifically for studies involving skin of marine fish. An apparatus was 3D-printed and consists of two plates, a lower and upper plate (with funnel). By mounting the explant between the plates, two chambers can be created with the

Last updated on: 04-02-2020 - 13:31

Organisation: Ghent University, Ghent University, Liège Université
Status: Internally validated