3D iPSC-chondrocyte model

Download PDF

Scope of the method

The Method relates to
  • Human health
The Method is situated in
  • Basic Research
  • Translational - Applied Research
Type of method
  • In vitro - Ex vivo
This method makes use of
  • Human derived cells / tissues / organs
Specify the type of cells/tissues/organs
3D IPSC-derived chondrocyte pellets/ IPSC-derived cartilage tissue

Description

Method keywords
  • IPSC reprogramming
  • IPSC-MC differentiation
  • flow cytometry
  • Chondrogenesis of iPSCs
  • immunohistochemical staining
  • qPCR assay
  • western blot
Scientific area keywords
  • Skeletal dysplasias
  • IPSC research
Method description
Aim: To model cartilage-related disorders in a human-specific context for the investigation of disease mechanisms and the screening of potential therapeutics. Technique: Fibroblasts and/or peripheral blood mononuclear cells (PBMCs) from both healthy donors and patients are reprogrammed into induced pluripotent stem cells (iPSCs). These iPSCs are first differentiated into mesenchymal-like cells and subsequently into 3D chondrocyte pellet cultures consisting of a mixture of both proliferating and hypertrophic chondrocytes similar to growth plate cartilage.
Lab equipment
  • - Laminar flow hood and incubator preferably in a dedicated stem cell lab.
  • - Flow cytometer to validate the mesenchymal-like cells for human mesenchymal stem cell markers.
  • - Real-Time PCR machine and immunostaining equipment for validation of the cartilage tissues.
Method status
  • Internally validated

Contact person

Pauline De Kinderen

Organisations

University of Antwerp (UAntwerpen)
Center of Medical Genetics
Belgium
Flemish Region