This method generates cortical brain organoids from embryonic stem cells or induced pluripotent stem cells. The protocol involves neural induction of 3D aggregates (embryoid bodies) which are then directed towards the cortical lineage in the presence of small molecules. SMAD inhibition (dorsomorphin

Colorectal cancer is the third most prevalent cancer worldwide, with radiotherapy being a common treatment. Existing models of the gastrointestinal tract, including mouse and 2D immortalized human cell culture models, lack the combination of human representability and radiotoxicity. This study seeks

We have developed a protocol for culturing primary neuron progenitor cells (NPCs) derived from mouse embryos at embryonic day 15 (E15). These cells are highly proliferative, can be subcultured and cryopreserved and can be differentiated to neurons or astrocytes. Hence, this method can greatly reduce