In silico tools are computer-assisted methodologies with a high-throughput that allow to predict the toxic potential of compounds without experimental testing. Consequently, in silico tools are time-, cost- and animal-saving in nature. The most commonly used methods are (quantitative) structure
Last updated on: 24-03-2022 - 11:25
The ATP cell viability assay (CellTiter-Glo assay test kit) provides a quantitative measurement of the ATP content directly proportional to the number of cells present in culture. The homogeneous assay procedure involves adding one single reagent, CellTiter-Glo Reagent directly to cells cultured in
Last updated on: 24-03-2022 - 11:20
Contact: Cannot be disclosed
Organisation: Sciensano
Status: History of use, Published in peer reviewed journal
We developed a high-throughput live-cell imaging-based organoid analysis platform, Organoid Brightfield Identification-based Therapy Screening (Orbits), that allows for kinetic monitoring of organoid growth and drug responses in routine extracellular matrix domes, high-throughput 384-well
Last updated on: 16-03-2022 - 13:59
Contact: Christophe Deben
Organisation: University of Antwerp (UAntwerpen)
Status: Internally validated
Quantitative Structure Activity Relationship modeling is generally used to construct models in which molecular descriptors of chemical compounds are used to predict endpoints/activities of interest. Commercial packages are available that can be implemented, but new models can be constructed if
Last updated on: 16-03-2022 - 13:49
Contact: Geert Verheyen
Organisation: Thomas More University of Applied Sciences
Status: Still in development, History of use, Published in peer reviewed journal
The multiplex immunoassay is based on the Luminex technology and allows the detection of diphtheria, tetanus and acellular pertussis antigens of human combined vaccines in the same run. As potency test of these vaccines are currently performed on animal through challenge and/or serological assays,
Last updated on: 28-02-2022 - 09:19
Maaike Vercauteren developed the innovative in vitro 'two-chamber skin explant model'. Pieces of skin are kept and examined in a controlled laboratory environment. This is no sinecure, because the skin must continue to function as if it were still attached to the fish. However, the tested setup
Last updated on: 22-02-2022 - 15:13
Contact: Cannot be disclosed
Organisation: Instituut voor Landbouw-, Visserij- en Voedingsonderzoek (ILVO), Ghent University (UGent)
Status: Internally validated
By using in vitro co-cultures of immune cells and lung tumor cells, we can study and evaluate the immune-activating and/or anti anti-tumor properties of certain treatments. Tumor cell proliferation can be studied with live-cell imaging instruments (IncuCyte, EVOS, ...) and the occurrence of immune
Last updated on: 21-02-2022 - 15:03
Contact: Eline Berghmans
Organisation: Vlaamse Instelling voor Technologisch Onderzoek (VITO)
Status: Published in peer reviewed journal
The in vitro rumen fermentation technique is used to simulate the rumen function/fermentation. The rumen is the first compartment of the digestive tract of ruminants, in which feed - consumed by ruminants - is fermented by rumen microbes. For optimal fermentation, the rumen provides an anaerobic
Last updated on: 14-10-2022 - 11:37
Contact: Veerle Fievez
Organisation: Ghent University (UGent)
Status: Published in peer reviewed journal
A set of protocols to combine the widely used Caco-2 cell line with digests from in vitro digestion models (small intestine, colon) to study toxicity, intestinal barrier integrity, bioavailability and, when combined with other cell models (immune, liver, endothelium), bioactivity of food related
Last updated on: 18-02-2022 - 08:42
Contact: Charlotte Grootaert
Organisation: Ghent University (UGent)
Status: History of use, Published in peer reviewed journal
Using respirometry (oxygen and pH, XF96 Analyzer), computer assisted cell analysis, and specific substrates and stressors, mitochondrial function and metabolic changes in a diverse set of cell lines can be measured. Relevant to study substrate preferences, acute and chronic effects of toxic
Last updated on: 15-02-2022 - 15:31
Contact: Charlotte Grootaert
Organisation: Ghent University (UGent)
Status: History of use, Published in peer reviewed journal
