HeLa cells are the first continuous cancer cell line and were isolated from the aggressive glandular cervical cancer of a 31-year old woman. It was the first aneuploid line derived from human tissue maintained in continuous cell culture. Knowledge of almost every process that occurs in human

Last updated on: 22-03-2019 - 11:39

Organisation: Vrije Universiteit Brussel
Status: Still in development, History of use

Human skin-derived adult stem cells differentiated towards hepatic cells (hSKP-HPC) are used in this method (R. M. Rodrigues et al., Stem Cells Dev. 23, 44–55 (2014)). These cells are exposed to a cocktail of insulin and glucose at certain concentrations. After 24h of exposure, these cells

Last updated on: 22-03-2019 - 11:39

Contact: Joost Boeckmans
Organisation: Vrije Universiteit Brussel
Status: Still in development

Non-alcoholic fatty liver disease (NAFLD) ranges from simple steatosis to severe, life-threatening non-alcoholic steatohepatitis (NASH). Steatosis is mostly asymptomatic and does not cause health complications. However, in 5-10% of the cases it proceeds to NASH in which hepatic inflammation

Last updated on: 22-03-2019 - 11:37

Contact: Joost Boeckmans
Organisation: Vrije Universiteit Brussel
Status: Still in development

The effect of fluidics that mimic the blood flow in the liver sinusoids, is evaluated during the hepatic differentiation of human skin-derived precursors (hSKP) [1],[2]. In a standard bi-dimensional (2D) cell culture system, hSKP are differentiated to hSKP-HPC for 24 days in static conditions. In

Last updated on: 22-03-2019 - 11:37

Organisation: Vrije Universiteit Brussel
Status: Still in development

For the generation of the human skin-derived precursors (hSKP) spheroids, ultra-low attachment 96-well plates, cell-repellent (Greiner) are used. hSKP cultured on tissue culture plates are detached with trypple (5 minutes) and collected in a tube. After centrifugation, hSKP are counted at the

Last updated on: 22-03-2019 - 11:36

Organisation: Vrije Universiteit Brussel
Status: Still in development

Drug-induced phospholipidosis (DIPL) is a metabolic disorder characterized by an excessive intracellular accumulation of phospholipids caused by cationic drugs [1]. Hepatic cells derived from human skin are evaluated as an in vitro model to investigate DIPL and its mechanisms. Human skin stem

Last updated on: 22-03-2019 - 11:36

Contact: Cannot be disclosed
Organisation: Vrije Universiteit Brussel
Partners: Cannot be disclosed
Status: History of use

This method describes a very reliable and robust in vitro model for the screening of the cholestatic liability of drugs and other chemical entities. The 3D spheroids generated from primary human hepatocytes can be cultivated up to 28 days, allowing long-term exposures which can depict otherwise

Last updated on: 22-03-2019 - 11:29

Organisation: Vrije Universiteit Brussel
Status: Still in development

The method detects two facets of drug-induced cytotoxicity i.e. the intracellular accumulation of phospholipids and of neutral lipids, i.e. phospholipidosis and steatosis respectively. The assay makes use of a kit containing an aqueous, red-fluorescent formulation of labelled phospholipids

Last updated on: 22-03-2019 - 11:29

Contact: Kaat Leroy
Organisation: Vrije Universiteit Brussel
Status: History of use